Applications Of Ionic Compounds With Fluorescent Propertie In Biometrics And Treatment Of Disease

Fluorescent probes,such as inorganic ions,quantum dots and organic materials,have been widely applied in detection,recognition and diagnosis with the technical progress of fluorescent probe,which bringing great benefits to our life and scientific research.However,with the going deep of the research work,there are always a few flaws like complex synthesis,poor solubility or stability,which limits the practical use of some probes.Ionic compounds exhibit good solubility and chemical stability,and also easily synthesized with good yields.Not only that there are special positive and negative charges in their structure,in particular cations,which could not merely recognize some negatively charged substance in living cells,but also could be applied in antisepsis or cancer therapy mediated by mitochondria.In this thesis,we combined“fluorescence”and“Ionic compounds”together,developing a series ionic fluorescent probes.In this way,the drawbacks of fluorescent probes were alleviated as well as the probes were applied in biomolecules recognition,antisepsis and anti-cancer treatments successfully.The thesis consists of four parts as following:1.A set of multiple cations doped linear polymers were synthesized via nucleophilic substitution between di-bromonated compounds and dipyridine derivative(OPY)which was prepared by by Suzuki coupling reaction.Due to the multiple cations existing in polymers,who can supply more opportunities for the connections with more anion,such as ATP,DNA,RNA and so on.therefore,alteration of conformation influenced the fluorescence signal.We synthesized fluorescent molecules,which could not only achieve specific recognition of ATP,but also be used as nuclear-targeting probes.2.A series of multiple cations charged AIE-active polymers(DBPE-DBO)were synthesized,which take tetraphenylethylene as their fluorescence core and bear long alkyl chain.Because of multiple positive charges on the polymer chains,DBPE-DBO possessed the multiple interaction points when contact occurs with the negatively charged bacterial cellular membrane or cell wall,and also,the hydrophobic alkyl chains were possibly able to intercalate into the membrane of the bacteria caused the nutriment could not enter into the cells of bacteria,thereby,achieving the sterilizing effect.Not only that,the long alkyl chain will interfere the permeability of bacterial cellular membrane and generate cell lysis,consequently,resulting the death of bacteria.The chemically modified reveals photodynamic therapy(PDT)effect,killing the bacteria further meanwhile reducing risks of drug resistance.Additionally,DBPE-DBO possessed multiple positive charges,which is able to contact with DNA resulting in a nucleus-target imaging of the bacteria.3.Ionic compounds play a positive role in cancer therapy.We synthesized a series of cations doped polymers taking tetraphenylethylene and triphenylamine as fluorescence core,they were named DPA-DBO-TPP,DBPE-2DBO-2TPP and BDPP-DBO-TPP.These polymers containing N~+and P~+.Due to the more negatively charged of membrane of cancer cells than ordinary cells,which could increase the targeting of cancer cells.The positively-charged polymers were permeated cancer cells and mainly centralized in mitochondria,disturbing the mitochondrial respiratory even destroying the mitochondrial membrane.This process could induce apoptosis through increase of ROS.