Distribution Metabolism And Biological Effects Of Two-dismensional Nanomaterials In Vivo

Two-dimensional(2D)sheet nanomaterials have unique properties in physics,chemistry,electricity and other fields,making them widely used in various fields.For example,in the biomedical field as a drug-loading platform,biosensors,biomedical imaging,stem cell technology and photothermal therapy.Significant changes have taken place in people's lives,bringing bright prospects to mankind.Graphene oxide is mainly composed of C,H and O.MoS2 is mainly composed of Mo and S.C,H,O,Mo and S are inseparable from these important elements in nature and in our daily life.Due to the outstanding advantages of the sheet nanomaterials,graphene oxide(GO)and molybdenum disulfide(MoS2),it is the most widely used 2D nanomaterial.Many reports have reported that MoS2 and GO are in the field of electronic information technology and medicine.Application in the field of defense.In particular,modified nanomaterials are widely used for drug delivery and medical imaging.Given its widespread use in popular areas,it is imperative to understand its biological properties and safety.In this paper,the graphene oxide was labeled with rare earth elements(La,Ce)(La/Ce-GO-PVP),which solved the problem that graphene oxide is difficult to quantify in vivo.The labeling rate and stability of La/Ce on GO were analyzed in detail by high performance liquid-inductively coupled plasma mass spectrometry(ICP-MS)and DLS.The size of the sheet was analyzed by transmission electron microscopy(TEM)and atomic-particle microscopy(AFM).CD-1 mice were injected with 200?L of 0.5 mg/mL La/Ce-GO-PVP(dose=5 mg/kg)in the tail vein,and their main organs were collected at different time points(4h,12h,24h,1week,4week).(Heart,liver,spleen,lung,kidney,brain,small intestine,testis,pancreas),digestion,analysis of La/Ce content by ICP-MS to quantify the distribution of GO in the body.Blood,urine and tissues were collected at different time points for pathological and pharmacokinetic studies.The results indicate that La/Ce-GO-PVP is mainly accumulated in the reticuloendothelial system and in the lung.The pharmacokinetics of La/Ce-GO-PVP.indicates that La/Ce-GO-PVP can be cleared rapidly by blood.In general,our data describe the biodistribution and biosafety of La/Ce-GO-PVP in vivo,making GO safer in biomedical applications.In order to improve the defect that MoS2 is plrone to precipitation under physiological system·we modified monolayer MoS2(PEG-MoS2)with isothiocyanate-polyethylene glycol(LA-PECG-NH2)to make it good dispersion in physiological solution.The physical and chemical properties were characterized by AFM,DLS and UV-vis.CD-1 mice were injected at a dose of 1.75 mg/kg.The tissue accumulation,metabolism,distribution,clearance and toxicological mechanism in the animals at different time points were analyzed.Our research indicates the biodistribution and biosafety of PEG-MoS2 in vivo.The research results in this paper provide useful data for the application of sheet nanomaterials GO and MoS2 in clinical medicine.