Functional MoS₂ As A Highly Effective Adsorbent For Uremic Toxin Removal

Objective:Uremia is a fatal syndrome that requires dialysis to treat.A wearable artificial kidney,if practical,could give patient continuous and convient dialysis resulting in better life quality and patient prognosis.However,currently available dialysis devices are too large to be put to practical application and achieve portable dialysis.Here we prepared nano-molybdenum disulfide,nano-molybdenum disulfide adsorbents with low cytotoxicity and high adsorption capacity,and we test their adsorption ability of urea and creatinine in pure water and ionic liquid respectively,and their cytotoxicity was determined in order to prepare a good nano-adsorbent system with high efficiency and low toxicity.Methods:1.Nano-molybdenum disulfide?MoS₂?was prepared by hydrothermal method,modified with mercaptoethylamine and carboxymethyl chitosan,finally get the carboxymethyl chitosan nano-molybdenum disulfide?CMC-MoS₂?adsorbent.Characterization ofcarboxymethyl chitosan nano-molybdenumdisulfidebyinfraredspectrumdetectionand analysis,UV-visible spectroscopy,X-ray photoelectron spectroscopy,scanning electron microscopy,Transmission Electron Microscope and X-ray diffraction.2.Adsorption isotherms and kinetic curves of adsorbents were measured to determinetheadsorptiveperformanceofadsorbents3.The cytotoxicity of HL7702?LO2?was measured by MTT assay.Results:1.MoS₂,NH₂-MoS₂ and CMC-MoS₂ were successfully prepared and identified by XPS,FTIR,TEM,XRD and SEM.2.Preparation of nano-adsorbent system.Under pure water and dialysate environment,the adsorption equilibrium time of N-CMC-MoS₂ and N,O-CMC-MoS₂ for urea is3 h.When urea concentration is 0.9 mg/mL,the maximum adsorption capacity of pure water is 500 mg/g,490 mg/g,and the maximum adsorption capacity of dialysate is 450 mg/g,250 mg/g.The adsorption equilibrium time of N,O-CMC-MoS₂ to creatinine was 1 hours,and the maximum adsorption capacity of dialysate was 200 mg/g.3.when the concentration of creatinine was 0.9mg/mL.In vitro toxicity study,when the concentration of CMC-MoS₂ incubated cells at 0-100 ug/mL,the increment rate of 7702 cells in normal human hepatocytes was more than 92%.4.In the stable assay,the nanoadsorbent show the great stability.Conclusions:CMC-MoS₂ has been shown to adsorb urea and creatinine,reaching a 20%removal efficiency from dialysis solution at the initial urea concentration of 30 mg/dL with the maximum urea adsorption capacity of 500mg/g and 100 mg/g.CMC-MoS₂ has a relatively high biocompatibility,resulting in a cell relative growth rate exceeding 80%at a concentration of 100?g/mL.Molybdenum disulfide was test to determine if it can become a new efficient portable adsorbent for dialysis.