Effect And Mechanism Of Betaine On Lipid Metabolism In Mice With High-fat Diet

Objective: to study the effect of betaine on lipid metabolism and its mechanism in obese mice with high-fat diet,and to lay a theoretical foundation for further study on the pharmacological effects of betaine and related health food research and development.Methods: 75 male kunming mice were bred adaptively for one week and then fed with high-fat diet to construct the model of obesity mice,which were divided into blank group(NC),blank + betaine group(NC-B),obesity model group(HFD),model + betaine group(HFD-B),model + simvastatin group(HFD-S).The normal saline solution of 0.25 ml 120mg/kg betaine was given to the NC group and HFD group at the same time every day,and the 80mg/kg sodium carboxymethyl cellulose solution of simvastatin was given to the HFD-S group at the same time every day.The relevant physiological and biochemical indexes were determined after 5 weeks of gavage.The contents of TC,TG,H-DLC,L-DLC,SOD,MDA and NEFA in mouse serum were detected by common biochemical kits.The contents of epinephrine,leptin and cAMP in mouse liver were detected by ELISA.Oil red O staining was used to observe the changes of lipid droplet area in the frozen section of the liver of mice,and HE staining was used to observe the changes of cell volume in the adipose tissue of mice.Expressions of AMPK,p-AMPK,HSL and p-HSL were detected by western-blot at protein level.Results: 1.Compared with the NC group,there were no significant differences in body weight,fat content and coefficient,liver weight and coefficient,and liver NEFA content in the NC-B group.Compared with the HFD group,the body weight,fat content and coefficient,liver weight and coefficient,and liver NEFA content in the HFD-B group and HFD-S group were significantly reduced.2.Serum test results of mice using common biochemical kits showed that: compared with the NC group,serum TG,TC,HDL-C andLDL-C levels of mice in the NC-B group showed no significant changes.Compared with the HFD group,the situation of blood lipid in the HFD-B group and the HFD-S group was effectively improved.3.The results of T-SOD and MDA kits showed that,compared with the NC group,the T-SOD content in the NC-B group increased significantly,while the MDA content decreased significantly.Compared with the HFD group,the content of T-SOD in the HFD-B group and the HFD-S group increased significantly,while the content of MDA decreased significantly.4.Oil red O staining results of frozen sections of liver tissue showed that compared with the NC group,the lipid drop area of the liver in the HFD group was significantly increased.Compared with the HFD group,the lipid drop area of the liver in the HFD-B group and the HFD-S group was significantly reduced.HE staining results of adipose tissue showed that compared with the NC group,the volume of adipose cells in the HFD group increased significantly,while the volume of adipose cells in the HFD-B group and HFD-S group decreased.5.Results of epinephrine,leptin and cAMP ELISA kit: compared with the NC group,the levels of epinephrine,cAMP and leptin in the HFD group were significantly decreased and the content of leptin was significantly increased.Compared with the HFD group,the levels of epinephrine,cAMP and leptin were significantly increased in the HFD-B group and the HFD-S group,while the leptin content was significantly decreased.Liver histone protein analysis results: compared with the NC group,the levels of AMPK,p-AMPK,HSL and p-HSL in the HFD group were significantly down-regulated.Compared with the HFD group,the expressions of AMPK,p-AMPK,HSL and p-HSL in mice of the HFD-B group and the HFD-S group were significantly increased.Conclusion: the results of this study showed that betaine can effectively reduce the body weight,liver weight and coefficient,fat content and coefficient in obese mice with high-fat diet.Improve the blood lipid status of obese mice,improve their antioxidant capacity,reduce lipid peroxidation;It can alleviate the accumulation of lipid in liver and inhibit the increase of fat cell volume.Leptinresistance was improved,adrenalin content was increased,and the expressions of cAMP,AMPK,p-AMPK,HSL and p-HSL in the liver were up-regulated.Therefore,betaine can regulate hormones such as leptin and epinephrine,activate AMPK signaling pathway,promote fat metabolism and improve obesity in obese mice,and preliminarily elucidate the mechanism of action.