Preparation Of Superhydrophobic-superhydrophilic Patterned Microchip By UV-induced Thiol-yne Click Reaction And Its Application

Superhydrophobic-superhydrophilic microchips are composed of superhydrophobicity and superhydrophilicity modes,which can control the distribution of droplets,reducing cross-contamination and improve the uniformity of analyte distribution.It has a wide range of applications in cell microarrays,DNA microarrays,protein microarrays and microarray-based detection of other molecules.The thiol-yne click reaction combines the typical characteristics of the click reaction with the advantages of photoinitiation.It is characterized by rapid reaction,mild conditions,and suitable for a variety of functional groups and used in chemical synthesis,space surface functionalization and biological conjugation.More importantly,photoinduced thiol-yne click reaction can achieve regional surface modification,which can produce superhydrophobic-superhydrophilic surface micropatterns.Combining the advantages of the click-reaction and superhydrophobic-superhydrophilic micropatterns,Based on the alkynyl film on the titanium sheet and glass substrate,the superhydrophobic and superhydrophilic micropattern was prepared by the thiol-yne click reaction.The whole preparation process only takes 25 min.The obtained micropattern has clear boundary between superhydrophobic and superhydrophilic region,strong mechanical resistance and good acid/alkaline resistance.Due to the difference in wettability between the superhydrophobic region and the superhydrophilic region,the droplets tend to be anchored in the superhydrophilic region and apart from the superhydrophobic region,which is benefit to reduce dosage of expensive reagents.The molecules will uniformly enrich in the superhydrophilic region during evaporation because of Malangoni effect.On this basis,a superhydrophobic-superhydrophilic microchip is developed.Combining with fluorescence imaging technology,the microchip can be used to detect O-phthalate in water,and the detection limit is as low as 10-7 mol/L.In addition,Taking advantage of the oxidative color rendering principle of glucose,the microchip can realize visual glucose detection within 15 min with the detection limit of 2 mM.