| With the deep intersection and rapid development of textile science and biomedicine,the application of textile materials in medical field has developed rapidly.Biomedical textile materials have many applications,especially in the field of tissue engineering.Biomedical textile materials play an irreplaceable role in the development and application of biomaterials.The inherent micro-scale fiber structure and the regular porous structure of textile materials make their surface topography very friendly to the biological environment.At present,the surface composition and structure of the fiber materials can be effectively regulated by various spinning and textile methods.It is also clearly recognized that the controllable micro/nano multilevel topology of the biomedical textile materials can affect the biological behavior of cells,and further guide the cells to achieve specific biological functions.The development of biomedical textile materials could provide an effective struetural template for investigating the response mechanism of cells to growth microenvironment,and provide a promising therapeutic approach for tissue regeneration and repair.In this dissertation,three kinds of silk fabrics with different fabric structures are obtained by means of textile:plain weave fabric(SFP),twill weave fabric(SFT)and satin weave silk(SFS).The SF/HA composite biomedical textile material with micro/nano multilevel structure was successfully prepared by using the alternating mineralization method and simulated body fluid(SBF)immersion method to introduce uniform nano-hydroxyapatite(HA)crystal coating on the surface of silk fabric.The effects of the fabric structure on cell adhesion,proliferation and migration behavior was studied.And the effects of nano-HA on the surface wettability,mechanical properties and cell compatibility of the fabric were investigated in order to achieve precise guidance and control of cell growth behavior.The main research results of this dissertation are outlined as following:(1)The cell growth behavior is guided and controlled by the three significantly different fabric structure of the silk fabrics SFP,SFT and SFS.The experimental results indicate that the fabric structure can effectively guide and control the cell adhesion area,adhesion elongation and orientation,and the cells show the most obvious anisotropic growth behavior on the satin fabric SFS.However,there was no significant difference in the proliferation behavior of cells on the three fabrics.This may be due to the fact that the basic structural units of the three fabries are silk fibroin fibers of about 10-14 micrometers in diameter,and the cell proliferation behavior is mainly related to the chemical composition of the fabric and the microfibrous structure.(2)The results of in vitro simulated wound healing experiment results indicate that the satin silk fabric(SFS)with significant anisotropic structure can enhance the guiding and controlling effect on the directional migration behavior of cells,and the healing of the wound model was completed on the SFS samples for 5 days.(3)A uniforn nano HA crystal coating can be successfully constructed on the surface of silk fabric by using alternating mineralization method and SBF immersion method.In the process of alternating mineralization,the pH value of P solution has a significant effect on the physicochemical properties of the coating.With the increase of the pH value of P solution,the particle size of ealcium phosphate salt in the coating is reduced to nanometer scale.The svurface of SF/HA composite fabric with micro/nano multilevel structure presented super hydrophilic property.In addition,the SF/HA composite fabric possesses a good biological activity.(4)The results of in vitro cell culture experiments indicate that the silk fabric still maintaines excellent biocompatibility after the introduction of nano-HA coating.Nano-HA combined with microscopic satin structure with significant anisotropy(sample SFS/HA)can cooperate to enhance the guiding and controlling effects on cell adhesion morphology and orientation growth along the microfiber. |
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