Effect Of The Lapsang Black Tea On Body Surface Electric Potential Difference And Primary Study Of Mechanism

Free radical is known as a perssad with unpaired electrons,its change in concentration can lead to the changes in cell membrane potential.Macroscopically,the effects of free radicals on cell potential can lead to the changes in bioelectricity,which are reflected and captured by the electro-physiological signals on body surface.Intake of antioxidants can scavenge excess free radicals in vivo.A large number of studies have shown that long-term intake of antioxidant-rich foods can improve oxidative stress and effectively reduce the incidence of disease.Besides the long-term effects,the short-term regulatory effect of antioxidant-rich foods has hardly been studied yet.Lapsang black tea was adopted as object in this study.Its antioxidant capacity was systematically investigated by various antioxidant activity assays,including ORAC,FRAP,ABTS and cellular antioxidant activity assay.The results showed that the antioxidant capacity of Lapsang tea in the intracellular and extracellular models was stronger than that of tea polyphenols and tea polysaccharide extract.In macrophages,the black tea can scavenge intracellular free radicals and change cell membrane potential.The interaction between black tea and macrophages,which derived from the mucosa of the digestive tract,suggests a new way for black tea to interact with human body in short term.The short-term regulatory effects of drinking Lapsang were also investigated in this study.Drinking tea can significantly down-regulate the levels of oral inflammatory factors such as IL-8,TNF-?,IFN-? and GCP-2 within 5 minutes.This short-term effect has the characteristics of rapid onset but short duration.Tea in the meal has a short-term regulating effect on blood sugar,which is manifested by the peak blood sugar level and the acceleration of blood sugar metabolism.Furthermore,because of the phenomenon that change of free radical concentration can affect electrophysiological signals on body surface,we also used electrophysiological methods to investigate the effect of Lapsang on human meridian electric potential difference(EPD).Drinking tea can significantly affect EPDs of heart meridian,stomach meridian and lung meridian in 5 minutes.Tea drinking in the meal has a significant effect on four meridian EPDs among the twelve meridians,including heart meridian,stomach meridian,gallbladder meridian and pericardium meridian.The effects of drinking tea on EPDs are not only reflected in the fluctuation range(voltage difference),but also in the fluctuation frequency.The effect of drinking black tea on the electrophysiological signals on the body surface may represent another way of food and body interaction.Food not only provides nutrition and energy,but also regulates the body's electrical system in an antioxidant manner.This mode of action is much faster than the process of digestion and absorption of food.It should be closely related to short-term regulation of antioxidant foods.There is a certain correlation between the effect of tea drinking on EPDs and the short-term regulation of blood sugar and inflammatory factors,but it is still unable to establish a reasonable correlation between them.As a methodological study in future,follow-up work needs to be further improved in terms of experimental methods and data mining.