Moderate Oxidative Stress Effect Of Black Tea And Theaflavins

With the popularity of tea customs, tea efficacy has been more and more attention. In this paper, the model organism Caenorhabditis elegans as a research object, exploring the mechanism of improving oxidative stress of black tea and its main functional components theaflavins under conditions of oxidative stress.Under normal culture conditions,500 and 1000mg/L black tea were significantly extend the average life of 8.1% and 9.4%(p<0.01).However,100 and 2000mg/L of black tea had no significant effect. Under oxidative stress,100,500 and 1000 mg/L black tea treatment group were significantly longer average life expectancy of 10.5%,18.1% and 24.2%(p<0.01). By detecting the effect on the growth and fecundity was found that 1000 and 2000mg/L black tea were not to change its development.Theaflavins improve oxidative stress in C. elegans is also present in a concentration differences. Under normal culture conditions,100 and 200mg/L Theaflavins respectively significantly extend the average life expectancy of nematodes 5.9%(p<0.05) and 8.2%(p <0.01). Under oxidative stress,100 and 200 mg/L theaflavins significantly extend the average life expectancy of 11.9% and 16.5%(p<0.01). However,50 and 400mg/L theaflavins showed no significant improvement (p<0.05) in life span of C. elegans and oxidative stress.100,200 and 400 mg/L theaflavins in the incubation 100-120 minutes can reduce oxygen content in vivo activity in C. elegans. Theaflavin will lose protection if knockout p38MAPK pathway sek-1 gene or pmk-1 gene of C. elegans. Theaflavin can regulate nuclear translocation of DAF-16. We found that the expression of antioxidase genes skn-1, sod-3, gsh-px, cat are upregulated under oxidative stress conditions.