Syntheis And Biomedical Properties Of Novel Hybrid Nanoparticles Based On Mesoporous Organosilica

Mesoporous organosilica nanoparticles(MONs)possesss typical controllable mesoporous structure,including uniform and tunable mesopores,large surface area and pore volume.Meanwhile,the biocompatibility of MONs is greatly improved by introducting organic groups,which is beneficial for lower hemolytic activity and cytotoxicity.In recent years,multi-functional nanosystems based on MONs have been widely studied in various biomedical applications incuding biomedical imaging,diagnostic and therapeutic applications.However,most of nanodrugs therapies are still severely limited far from clinical trials.The main problems are:low circulation time of nanoparticles in the blood,the physiological barrier in vivo which hinder the accumulation of the nanoparticles in tumor areas,and the biosafety of the nanoparticles.Enhancing tumor cellular uptake efficiency is one of the effective methods for enhancing relative accumulation of nanoparticles in tumor areas.Based on the above facts and problems,to enhance the cellular uptake of tumor cells,increase the accumulation of nanoparticles in the tumor areas and accumulate the delivery of drug in the deeper tumor areas,a series of works are carried out as followed:(1)Soft mesoporous organosilicon nanorods were prepared by dispersive-protection etching method,in which gold nanorods(GNR)was used as a template for geometric morphology regulation.Polyethyleneimine was used as the protective layer of highly dispersed particles.The results show that the soft mesoporous nanorods possess uniform mesoporous(3.9 nm),high specific surface area(355 cm~2/g)and large pore volume(0.35 cm~3/g).It is worth noting that the soft mesoporous nanorods have good biocompatibility after HA modificaiton.In addition,the soft mesoporous nanorods have achieved 2-fold cellular uptake efficiency comparing with the hard counterparts.(2)The mesoporous organosilica nanomotors were prepared by aqueous synthesis method.The migration rate of the prepared nanomotors is measured to be significantly enhanced in the presence of hydrogen peroxide.In addition,the prepared nanomotors possess preferential moving towards solutions which containing hydrogen peroxide,which is valuable and promising in biomedical appilications of drug delivery and enhanced-tumour-permeability,as the content of hydrogen peroxide in tumor cells is generally higher than that in normal cells.