Study Of The Detection Of Microcystins On Novel Three-Dimensional Paper Microfluidic Chip Base On Molecular Imprinting

The detection of microcystins,a toxic substance produced by the eutrophication of water,is becoming more and more important.Microfluidic paper chip is a new field that has been developed in recent decades,which provide a good platform for the detection of microcystins.In this paper,microcystins in water were detected on a new three-dimensional microfluidic paper chip by means of quantum dots and zinc ferrite catalysis.The main research contents and results are as follows:1.Simple Way To Fabricate Novel Paper-Based Valves Using Plastic Comb Binding SpinesA new type of three-dimensional microfluidic paper chip using plastic comb binding spines was tested for water pollutants.The fluid and flow rate of the paper-based equipment were investigated.The ferrous ions and nitrite were tested on paper-based equipment,and the detection limits were 8.9 mg/L and 0.28 mg/L,respectively.The recovery rates of ferrous ions and nitrite in water samples were 103.9%¹05.3%and104.1%¹08.0%,respectively.It is concluded that the novel three-dimensional microfluidic paper chip has good performance,low production cost,simple operation,and can be used for various aspects such as water pollutants.The novel three-dimensional microfluidic paper chip has the advantages of good performance,low production cost,simple operation,and can be used for detecting various aspects such as water pollutants.2.Detection of microcystins on novel three-dimensional microfluidic paper chips based on quantum dot and molecular imprinting.The cadmium telluride quantum dots were grafted on the glass fiber paper chip,and the arginine molecularly imprinted polymer was synthesized on the surface of the quantum dots by the virtual template technique.The template was eluted and used for the detection of microcystins in the water.The paper chips of cadmium telluride quantum dots and grafted molecularly imprinted polymers were characterized.The conditionsaffecting the experiment:pH,APTES dosage,elution time and adsorption time were investigated.Fluorescence spectrum curves and standards were obtained by static adsorption,LOD=3.6?g/L.The stability and selectivity of the paper chip were investigated.The paper chip was used for the detection of actual lake water and seawater,the recovery rates were 98.4%¹04.8%and 101.5%¹05.13%,respectively.It is concluded that this method has high sensitivity,selectivity,practicability,and can be used for the detection of microcystins in actual water bodies.3.Detection of microcystins on novel three-dimensional microfluidic paper chips based on zinc ferrite catalysis and molecular imprinting.The zinc ferrite catalytic material was synthesized and coated on the surface of the silicon.On the basis of this,an arginine molecularly imprinted polymer was synthesized,and the molecularly imprinted polymer was used to catalysis H₂0₂ and TMB on the novel three-dimensional microfluidic paper chip.The microcystins are detected by the relationship between the color intensity of the reaction and the amount of the adsorbed target.The standards curve was obtained by color reaction LOD=3.4?g/L.The selectivity of the paper chip was examined.The paper chip was used for the detection microcystins of actual lake water and seawater,and the recovery rates were92.4%¹07.7%and 94.2%¹02.8%,respectively.It is concluded that this method has excellent performance,strong selectivity and high availability,and can be used for the detection of microcystins in actual water bodies.