Study On Separation Analysis And Origin Identification Of Wine

In this paper,the application and research progress of mineral element?inorganic element?analysis,stable isotope analysis and infrared spectrum analysis in the field of wine origin traceability are introduced.The effects of electrophoretic medium,concentration of electrophoretic buffer solution,pH value of buffer solution,separation voltage and detection wavelength on the separation and detection of wine by capillary electrophoresis were explored,and the separation and detection method of wine by capillary electrophoresis was established.Thirty-five kinds of wine were separated and detected.the capillary electrophoresis fingerprint of wine was established according to the migration time of12 common peaks,which can be used for wine identification and quality control.Principal component analysis and linear discriminant analysis were carried out on12 peak heights of 35 wines by capillary electrophoresis.the wines of the United States,Chile and Spain were distinguished by principal component analysis.Wines from France,Italy and Australia,on the other hand,do not distinguish well.Linear discriminant analysis can be used to distinguish wine from France,the United States,Italy,Australia,Spain and Chile,and the correct rate of discrimination is 88.6%.The contents of ethanol?¹³C,glycerol?¹³C,?¹⁸O and Ca,K,Mg,Na,B,Al,Sc,Ti,Cr,Mn,Fe,Cu,Zn,Rb,Sr and Ba in 85 wines were determined.The stable isotopes and inorganic elements of wine were analyzed by regional difference analysis,discriminant analysis and principal component analysis.It was found that there was no significant difference in ethanol?¹³C and glycerol?¹³C among wines from different producing areas,but there was no significant difference in ethanol?¹³C and glycerol?¹³C among wines from France,Australia,South Africa and Chile,but there was a linear correlation between ethanol?¹³C and glycerol?¹³C.However,?¹⁸O of wine from different producing areas was significantly different.Except K,Mn,Cu,Zn and Ba,the contents of Ca,Mg,Na,B,Al,Sc,Ti,Cr,Fe,Rb and Sr in wines from different producing areas were significantly different.In particular,the contents of Na,B,Al,Sc,Ti,Fe,Rb,Sr and other elements were significantly different.The discriminant analysis and principal component analysis of stable isotopes and inorganic elements in wine were carried out.It was found that the stable isotopes and inorganic elements in wine had strong regional characteristics.It contains the vast majority of regional characteristics of all stable isotopes and inorganic elements.The correct identification of wine producing area can be obtained by discriminant analysis based on the data of characteristic stable isotopes and inorganic elements.The correct rate of discrimination is high,up to 96.7%,and the result is satisfactory.On the other hand,the principal component analysis can distinguish the wines of France and Australia,but the classification results of South Africa and Chile are not satisfactory.When principal component analysis is carried out with characteristic stable isotopes and inorganic elements,the effect of dimensionality reduction is better,and fewer principal components can be used instead of the original index for wine origin classification.