Preparation And Application Of Aggregation Induced Emission Nanoparticles And Test Strip Detection System

Luminescent materials with aggregated induced emission(AIE)properties have attracted extensive interest and are considered as a novel class of luminogens to develop fluorescent sensors with superior sensitivity.It can overcome the disadvantage of traditional fluorescent materials that aggregation cause quenching and possesses good photobleaching resistance.It is a kind of superior fluorescent material.Herein,we have designed two AIE fluorescence detection systems for the detection of carboxylesterase and phosgene,respectively.1.A ratiometric AIE nano-fluorescence sensing system was constructed for the detection of carboxylesterase(CaE)in serum:Carboxylesterase(CaE)is a class of enzymes used to catalyze the hydrolysis of fatty acid esters to acids and alcohols.Human plasma carboxylesterase may be a new serum biomarker for hepatocellular carcinoma.Therefore,the establishment of a reliable serum CaE fluorescence detection system is of great significance in clinical application.In this paper,TPE-N+and fluorescein diacetate(FDA)were prepared,which constitute the detection system.The chemical structures of TPE-N+and FDA were characterized by ~1H NMR and MS.Absorption and fluorescence spectra were used to investigate the spectral properties,time response,concentration response,selectivity of the detection system and its application in real samples.The energy donor of the detection system possesses good AIE properties.The detection system has a low detection limit(0.26 U/L).Its fluorescence emission peak red shifts after the reaction with phosgene,so it can achieve sensitive and highly selective detection of CaE in ratiometric way and can be applied to human serum samples.2.A ratiometric AIE solid-state fluorescence sensor was constructed for the detection of phosgene:Phosgene is a kind of colorless toxic gas.Inhalation of phosgene can lead to severe acute respiratory problems,including non-cardiogenic pulmonary edema,emphysema,etc.It can even lead to death in severe cases.Therefore,it is of great significance to realize the fast and convenient detection for phosgene.In this paper,we designed and synthesized the probe OPD-TPE-Py-2CN.The chemical structure of OPD-TPE-Py-2CN was characterized by ~1H NMR and MS.OPD-TPE-Py-2CN was deposited on cellulose filter paper to obtain phosgene test strips.Absorption and fluorescence spectra were used to investigate the spectral properties of the test strips and its response to phosgene,selectivity and anti-interference.The recognition mechanism of the test strips toward phosgene was investigated by ~1H NMR and MS.The probe exhibits good AIE properties.The test strips response to phosgene rapidly and has a low detection limit(1.87 ppm).After reaction,the fluorescence emission peak red shifts.Thus,the test strips can achieve rapid and highly selective detection of phosgene in ratiometric way.