Bioaugmentation With Two Strains Of Comonas Testosterone Towards Quinoline And Phenol Removal In Coking And Dyeing Wastewater Treatment

Bioaugmentation of refractory wastewater was a hot-spot in the field of environmental pollution control.The refractory organics in wastewater could be effectively biodegraded by adding special cultured bacteria with high environmental adaptability and rapid proliferation.Herein,bioaugmentation of dyeing wastewater and coking wastewater was conducted by adding cultured two strains of comonas testosterone which had ability of proliferation using refractory compounds as nutrient.The main results were listed below.(1)Polyether sulfone(PES)beads were synthesized to load the strains for improving the shock resistance of the strains in treating of dyeing wastewater,using polyurethane(PU)foam and free stains as reference.The results show that uniform beads with diameter of 1-3 mm was prepared via a facile vapor phase separation route.The pore size in beads was enlarged with the increase of dripping time through the water vapor cylinder,and optimized to 3?m when the cylinder was 2.5 m in length.Thus,the PES beads provided desirable surface pore for bacteria to pass through and grow inside.In addition,the PES beads had high special surface area of 1843 cm~2/cm~3 and could load 0.024 g dry mass/cm~3 of C.testosterone QYY in comparison with the polyurethane foam.The reactor with the loaded C.testosterone QYY on PES beads showed well shock-resistance and optimal total organic carbon(TOC)removal,which had 100%removal efficiencies of both quinoline and phenol when the volume ratio of the dyeing wastewater to domestic wastewater was elevated from 1:2 to 1:1,in comparison with the 100%and 34.7%removal rates by the reactor with immobilized C.testosterone QYY on PU foam,or the 82%and 2.4%removal rates by the reactor with free C.testosterone QYY.The loaded C.testosterone QYY on PES beads demonstrated good shock resistance and was able to completely degrade the toxic compounds,such as phenyl carbamate,2-nitrotoluene and dioctyl phthalate.(2)A new C.testosterone bdq06 was directly inoculated in active sludge system for treating dyeing wastewater with the addition of domestic wastewater as nutrient.The results show that C.testosterone bdq06 proliferated naturally with adding only once.Subsequently,the reactor with adding C.testosterone bdq06 exhibited well shock-resistance,in which approximately 85.1%of TOC was effective removed and no shock was observed from increasing the organic loadings.Conversely,the reactor without adding C.testosterone bdq06demonstrated that TOC removal was 11.1%less and the recovery of the shock was about 12days.The addition of C.testosterone bdq06 enhanced complete degradation of quinoline,phenol and other refractory compounds in the wastewater.(3)Coking wastewater was effectively treated by inoculating C.testosterone bdq06 in a micro-pressure swirl reactor which generated an external aerobic zone and an internal anaerobic zone for simultaneous denitrification.Higher efficiencies of 90%TOC,92%ammonia and 78%nitrate removal were achieved by the bioaugmentation comparing to the control and insignificant shocks were observed from increasing the organic loadings.High throughput sequencing result showed that the inoculated C.testosterone bdq06 adapted and proliferated in the processes,and predominated for phenol and quinoline degradation.