| In recent years,the treatment of difficult diseases such as cerebral infarction,cerebral hemorrhage,diabetes and arteriosclerosis,which have high incidence,mortality and disability,has become a major medical problem.Related reports indicate that this has a lot to do with the content of H2O2.When the H2O2 content in the body is too high,it means that the oxidation and anti-oxidation effects in the body are imbalanced,and a large amount of oxidation intermediates are generated.The accumulation of H2O2 can cause serious damage to cells,destroy lysosomal membranes,cause cell death,etc.[1,2].Therefore,it is necessary to carry out strict monitoring of the H2O2 content in the body,and it is urgent to study the H2O2 biosensor which can meet the detection requirements and be detected in real time.This paper requires the design of a hydrogen peroxide sensor suitable for integrated use.In view of the limitations of the use of the scene,this paper used screen printing technology to prepare the base electrode,pulse electrodeposited Nano-Au particles to modify the working electrode.In the process of optimizing the electrode,we produced laser-induced graphene through a new material,Nomex Paper,which improved the performance of printed electrodes and enabled a wider range of more accurate measurements of H2O2 in electrolyte solutions.The main work content can be divided into the following four parts:?1?The conductive load substrate electrode was prepared by screen printing using carbon pastes CH-8?MOD2?from Japan,and modified by pulse electrodeposition Nano-Au.The pulse electrodeposition parameters are a forward current density of 12 mA/cm2 and a negative current density of 625 mA/cm2,pulse period is 2mS,duty cycle is 50% and deposition time is 1min.The electrochemical performance of the modified working electrode in hydrogen peroxide solution was measured and analyzed.The linear detection range is4.0×10-7 mol/L2.6028×10-3 mol/L,and the lowest detection limit is up to 2.0×10-7 mol/L.?2?Based on CH-8?MOD2?electrode,we further optimized and upgraded the conductivity and load capacity of the substrate electrode.Laser-induced graphene was prepared using Nomex paper which has excellent conductivity and 3D foam porous structure.Doping laser-induced graphene into CH-8?MOD2?conductive carbon paste greatly reduces the electrode resistance and increases the adhesion specific surface area of Nano-Au.The final linear range of the sensor is 1.0×10-7 mol/L3.4554×10-2 mol/L.The sensitivity of the detection is as low as 1.0×10-8mol/L.The anti-interference test of the sensor was carried out by using glucose,alanine,arginine and ascorbic acid,which proved that the anti-interference performance of the sensor was good.?3?In order to better integrate the sensor into the microfluidic system,we designed a multi-channel microfluidic electrode.In view of its operability,ease of assembly,non-toxicity and compatibility to cells,PDMS was used to make microfluidic chip.The chip was built with a microfluidic loop measurement system to further study the performance of the electrode in the microfluidic chip.A certain amount of hydrogen peroxide was dropped into the medium to simulate the cell environment,and the error analysis of the sensor was made according to the measurement result.?4?Finally we summarized the content of this paper,reflected on its own shortcomings and planned future research content and direction. |
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