Study On The Polysaccharides Extraction,Antioxidant Properties Of Tribonema Sp. And Its Application In The Treatment Of Food & Chemical Industry Wastewater

Filamentous microalgae,which is easy to harvest and resistant to rotifer pollution,is a new research focuse in the field of microalgae engineering.In this paper,an high sugar-containing Tribonema sp.was study,optimizing the extraction technology of Tribonema sp.polysaccharides and preliminarily studying the physical and chemical properties and antioxidant activity of crude polysaccharide.Furthermore,this strain was applied to the wastewater treatment of food chemical industry park,and the biomass of high sugar Tribonema sp.was obtained when the wastewater was purified.After 9 days of cultivation for Tribonema sp.,the biomass were up to 4.25 g/L and 50%under the condition of 2%CO₂ bubbling,respectively.The extraction process of polysaccharides was optimized by hot water extraction-ethanol sedimentation method.The optimum conditions were temperature 80?,time 2 h,pH9,ratio of water to materiel 0.5:1 mL/mg,and the yield could reach to 27.25%.The crude polysaccharide was separated by DEAE-cellulose anionic column,only one main peak.After,the crude polysaccharide was used as the research object,it was composed of rhamnose,arabinose,xylose and mannose and the molar ratio is 0.31:0.26:0.40:0.42:1.00:0.47,the uronic content acid was 14.57%and sulfate content was 42.21 mg/g.The Infrared spectroscopy showed that the polysaccharides of Tribonema.sp exhibited typical absorption peaks,which were?-glycosidic bond configuration.In addition,Tribonema sp.polysaccharides had a certain vitro antioxidant activity,in the 0².0 mg/L of seting concentration range,the scavenge effect and the reducing force are both improved with the increase of the concentration of the Tribonema sp.polysaccharides,and the dose effect was presented.The scavenge rate of the superoxide radical reached to 69.52%when the polysaccharide concentration was 1.0 mg/mL,and the scavenge rate of the hydroxyl radical was61.89%at the concentration of 0.6 mg/mL.Tribonema sp.was cultured in the artificial wastewater from food flavor production and the real industrial wastewater from food chemical industrial park.The results showed that the biomass of the experiment group,which was the initial concentration of 100 mg/L acetophenone under the condition of 2%CO₂ bubbling,was up to 4.0 g/L,the CO₂ bubbling could improve the tolerance of Tribonema.sp to the acetophenone,and basically did not influence the material composition of Tribonema sp.cell content.After 9 days of treatment,acetophenone was completely removed from the experimental group which initial concentration of acetophenone was less than 200 mg/L.The degradation process of the acetophenone was also analyzed,inferingthatthe acetophenonemaybefirstconvertedinto?-methyl-benzenemethanol,and then converted into phenol,and finally the acetophenone was completely degraded.In the comprehensive wastewater from food chemical industrial park,the Tribonema sp.was combined with the anaerobic/aerobic?A/O?process of the traditional wastewater treatment,and the wastewater in the three stages of the primary clarification tank,the anaerobic and anaerobic/aerobic stage was selected as the culture medium of Tribonema sp..The results indicated that the anaerobic effluent has the best growth state and wastewater treatment effect,the biomass,COD and the total nitrogen removal rate were 4.4 g/L,98.4%and 96.8%,respectively.The sugar content of Tribonema sp.was as high as 49.6%,the oil content was 36.1%,the C16:1?palmitoleic acid?accounted for about 50%of the total fatty acid.The combination of the cultivation of Tribonema.sp and the traditional anaerobic effluent treatment was an innovation method which could effectively reduce the energy input of the aerobic aeration,realizing the resource utilization of the wastewater,and simultaneously obtaining the high-sugar algae biomass for development and utilization in the fields of food,medicine,energy,chemical industry.