| Because of accidents,bone tissue lesions and age growing,the number of orthopedic diseases accounted for 5% of the world's population.The number of death caused by this such diseases are countless.With the development of the biomedical engineering,the requirements of biomedical materials are getting higher and higher.Scientists are constantly trying to develop new materials to meet the needs of patients,to achieve the purpose of treatment and cure.Being one of the three elements of tissue engineering,three-dimensional porous structure scaffold plays an important role in tissue engineering.As it not only provide cells for the life,but also serves as a template to guide tissue regeneration and control of organizational structure and other functions.Hyaluronic acid(HA),a kind of natural polymer,is one of the main components of articular cartilage,but also an important component of synovial fluid,which plays an important role in the nutrition and metabolism.In addition to providing bone marrow stem cell adhesion and tissue replacement of micro-environment,but also play a bone induction.Based on these unique functions,hyaluronic acid is widely used in tissue engineering.However,hyaluronic acid cannot separately used in bone tissue scaffolds.Because of its viscosity and mechanical strength is not enough,and the degradation rate is faster,it is not easy to maintain a certain three-dimensional structure,and can not provide a good guarantee for the maintenance of bone cell for the growth.In order to solve these problems,this study introduced gelatin and ?-TCP to prepare composite scaffolds.Gelatin,as one of the degradation products of collagen,has a lower antigenicity than collagen and retains its amino acid sequence and biological activity,enhancing cell adhesion activity.Because of good biodegradability and biological activity,gelatin is usually mixed with other biological materials to prepare three-dimensional porous scaffold in the tissue engineering,which has been applied.in the skin,nerve,bone tissue engineering.?-TCP is a biocompatible biodegradable biologically active ceramic that can be degraded in vivo and in a cell-mediated manner.Its degradation products can participate in life activities and metabolism and become part of a living tissue.The material has good biocompatibility and cartilage tissue growth in vivo.In this study,1-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride(EDC)was used as crosslinking agent to crosslink hyaluronic acid with gelatin,and then composite ?-TCP microspheres to prepara porous hydrogel scaffolds.The microspheres were analyzed by X-ray diffraction(XRD).The scaffolds were characterized by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR).At the same time the compressive strength,swelling ratio,degradation of the scaffold material are also measured.To assess the cell compatibility of the stent in vitro,mouse L929 fibroblasts were seeded onto scaffolds for cell morphology and cell viability studies.The results show that the pore size of the porous scaffold can be controlled by changing the ratio of gelatin to hyaluronic acid,and the diameter of the hole would increase with the proportion of hyaluronic acid increased within a certain range.With the increase of the proportion of hyaluronic acid in the mixed stent,the swelling ratio and the degradation rate also increased.The compressive strength of the stent increases with the increase of the proportion of gelatin.A certain amount of ?-TCP can promote cell growth and proliferation. |
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