Study Of Bioglass/Gelatin/Collagen Composite Scaffold

In this work,acid-pepsin solubilized collagen was extracted from fresh porcine skin from local market.Polyacrylamide gel electrophoresis?SDS-PAGE?,Fourier transform infrared spectrometer?FTIR?,and differential scanning calorimetry?DSC?were used to study the structure of the collagen.SDS-PAGE showed that there were?,?₁ and?₂ chains.The extracted product was proved to be type I collagen by infrared spectroscopy.Bioactive glass was prepared by a sol-gel method.FTIR and particle size analysis were used to study the prepared bioactive glass.The Si-O-Si asymmetric vibration peak appeared at the 1050cm^-1,the Si-O-H symmetrical vibration peak appeared at 815 cm^-1 and the bending vibration peak appeared at 475cm^-1 in the FTIR spectra.The bending vibration peaks of the amorphous p-o groups were found at the606cm^-1 and 560cm^-1.They are the infrared characteristic peaks of bioactive glass.The particle size of 45S5-type bioactive glass was about 16?m.3-propyl tri-ethyl siloxane?APTES?was used to modify the bioactive glass.The amino group on the surface of bioactive glass was modified by coupling chemical reaction.FTIR and particle size analysis were used to study the modified bioactive glass.Both the characteristic peaks of N-H at 695cm^-11 and the N-H bending vibration peak at 1550cm^-11 indicated the existence of amino groups on the surface of modified bioactive glass.Particle size analysis showed that the particle size of the 45S5 was about 18?m after the modification.The Zeta potential was the greatest at the APTES dosage of 3%?v/v?,23m V.The bioactive glass/gelatin/collagen composite scaffolds were prepared with modified bioactive glass,gelatin and collagen.The compressive stress of the composite was 5.97 MPa at the deformation of 50%,which was increased slightly to6.24MPa after amino-modification.FTIR spectra indicated the formation of new amide bonds between amino-modified bioactive glass and gelatin or collagen,which enhanced the structure stability of the resultant composite.The bioactive glass/gelatin/collagen composite scaffolds were immersed in simulated body fluids for mineralization,and hydroxyapatite was observed on the surface of scaffolds by SEM images.The hydroxyapatite granules increased with extending the mineralization time.The bioactive glass/gelatin/collagen composites were immersed in phosphate buffer?PBS?for degradation.After 28 days,25.25%in weight was kept.In case of amino-modified bioactive glass/gelatin/collagen composite,32.96%was kept,indicating that amino-modification could improve the interfacial compatibility between inorganic and organic phases,thereby improve the structure stability of the composite.According to relative growth rate and toxicity grade,the toxicity of amino-bioactive glass/gelatin/collagen composite scaffold was classified as grade 0 or1.Therefore,it is expected to be used in bone tissue engineering repairing.