Studies On Separation And Purification Of Chemical Constituents From Traditional Chinese Medicine Inonotus Obliquus ,Clinacanthus Nutansae And So On

Due to the complex composition of Chinese medicine and the low content of active ingredients,which limits its effective use and development,it is especially important to establish a convenient and effective method for separation and purification of traditional Chinese medicine.In this paper,high-performance liquid chromatography was established for the separation and purification of chemical constituents from Chinese herbal medicines such as Inonotus obliquus,Clinacanthus nutans,Marsdenia tenocissima,Kadsura coccinea and Citrus reticulata Blanco were established.The analytical method was carried out,and the purification process of chemical components was studied by macroporous adsorption resin,which established a convenient and efficient purification method.1.Isolation and purification of chemical components of Inonotus obliquusThe extraction process of the chemical components of Inonotus obliquus was optimized.The best extraction process was as follows:95%ethanol was extracted twice under heating and reflux for each time 30 min,and the ratio of material to liquid was 1:20.The purification process of triterpenoids from Inonotus obliquus was studied by macroporous adsorption resin method.The amount of triterpenoids in Inonotus obliquus was used as an indicator to compare the adsorption and desorption capacities of the 5macroporous resins for triterpenoids from Inonotus obliquus,and the preferred resin was optimized for process parameters.The results show that the D101 macroporous resin has good adsorption and desorption properties,and the adsorption behavior is more consistent with the Langmuir model.The optimized conditions were as follows:the sample amount was 5 BV,the sample concentration was 4 mg·mL^-1,and removing impurities with 10 BV40%ethanol solution,the eluent was 95%ethanol solution,the flow rate was 2 mL·min^-1,the volume of elution was 7 BV.The content of triterpenoids in Inonotus obliquus was increased from 25.68%to 82.00%after purification of the resin.It was confirmed that the method established in this experiment can effectively purify the triterpenoids of Inonotus obliquus.2.Isolation and purification of chemical components of Clinacanthus nutansThe purification process of flavonoids from Clinacanthus nutans was studied by macroporous adsorption resin method.The amount of flavonoids in Clinacanthus nutans was used as an indicator to compare the adsorption and desorption capacities of the 7macroporous resins for flavonoids from Clinacanthus nutans,and the preferred resin was optimized for process parameters.The results show that the HPD-100 macroporous resin has good adsorption and desorption properties,and the adsorption behavior is more consistent with the Langmuir model.The optimized conditions were as follows:the sample amount was 6 BV,the sample concentration was 10 mg·mL^-1,and removing impurities with 10 BV distilled water,the eluent was 40%ethanol solution,the flow rate was 4mL·min^-1,the volume of elution was 7 BV.The content of flavonoids in Clinacanthus nutans was increased from 61.28%to 91.74%after purification of the resin.It was confirmed that the method established in this experiment can effectively purify the flavonoids in Clinacanthus nutans.3.Isolation and purification of chemical components of Marsdenia tenocissimaThe purification process of C₂₁1 steroidal saponins from Marsdenia tenocissima was studied by macroporous adsorption resin method.The amount of C₂₁1 steroidal saponins was used as an indicator to compare the adsorption and desorption capacities of the 7macroporous resins for C₂₁1 steroidal saponins from Marsdenia tenocissima,and the preferred resin was optimized for process parameters.The results show that the AB-8macroporous resin has good adsorption and desorption properties,and the adsorption behavior is more consistent with the Langmuir model.The optimized conditions were as follows:the sample amount was 8 BV,the sample concentration was 10 mg·mL^-1,and removing impurities with 10 BV 20%ethanol solution,the eluent was 80%ethanol solution,the flow rate was 4 mL·min^-1,the volume of elution was 7 BV.The content of C₂₁steroidal saponins in Marsdenia tenocissima was increased from 42.18%to 88.59%after purification of the resin.It was confirmed that the method established in this experiment can effectively purify the C₂₁1 steroidal saponins of Marsdenia tenocissima.4.Isolation and purification of chemical components of Kadsura coccineaThe purification process of the chemical components of Kadsura coccinea was studied by macroporous adsorption resin method.The adsorption and desorption capacities of the 7 macroporous resins were compared,and the preferred resin was optimized for process parameters.The results show that the HPD-400 macroporous resin has good adsorption and desorption properties,and the adsorption behavior is more consistent with the Langmuir model.The optimized conditions were as follows:the sample amount was 6BV,the sample concentration was 10 mg·mL^-1,and removing impurities with 10 BV 20%ethanol solution,eluted with 80 BV 40%ethanol and 5 BV 95%ethanol,respectively.The flow rate was 4 mL·min^-1.The content of lignans in Kadsura coccinea was increased from20.87%to 88.41%and the content of tritepenoidic acid in Kadsura coccinea was increased from 11.49%to 81.05%after purification of the resin.It was confirmed that the method established in this experiment can effectively purify the chemical components of Kadsura coccinea.5.Isolation and purification of chemical components of Citrus reticulata BlancoThe purification process of flavonoids from Citrus reticulata Blanco was studied by macroporous adsorption resin method.The amount of flavonoids in Citrus reticulata Blanco was used as an indicator to compare the adsorption and desorption capacities of the7 macroporous resins for flavonoids from Citrus reticulata Blanco,and the preferred resin was optimized for process parameters.The results show that the HPD-400 macroporous resin has good adsorption and desorption properties,and the adsorption behavior is more consistent with the Langmuir model.The optimized conditions were as follows:the sample amount was 5 BV,the sample concentration was 80 mg·mL^-1,and removing impurities with 10 BV distilled water,the eluent was 80%ethanol solution,the flow rate was 4mL·min^-1,the volume of elution was 8 BV.The content of flavonoids in Citrus reticulata Blanco was increased from 42.18%to 88.59%after purification of the resin.It was confirmed that the method established in this experiment can effectively purify the flavonoids of Citrus reticulata Blanco.