Chiral Mesoporous Silica Used As Stationary Phases In High Performance Liquid Chromatography

In this paper,four chiral mesoporous silica columns were studied.The main contents include the following:First,chirality,optical resolution,separation method,enantioselective stationary phase,and chiral high performance liquid chromatography?HPLC?were introduced.Preparation and application of chiral mesoporous silicon materials were briefly described.Secondly,the hexagonal prismatic chiral mesoporous silicon?CMS₁?was prepared by soft template method using C₁₄-D-Phe anionic surfactant synthesized under certain conditions as template.The chromatographic column was applied to HPLC to separate chiral compounds and benzene positional isomers.The experimental results show that the CMS₁ column has recognition characteristic on the chiral compound and positional isomer,and its stability is good,so the material can be used for chiral resolution.Thirdly,the C₁₄-L-Phg anionic surfactant synthesized by L-phenylglycine was used to prepare chiral mesoporous silicon.The chromatographic column could separate chiral compounds and benzene-based positional isomers.The effects of temperature,injection volume and other factors on the column were also investigated.Fourthly,the chiral mesoporous silicon from anionic surfactant C₁₄-L-Pro was synthesized to prepare chiral column,this HPLC column possessed enantioselective ability.Finally,according to above method,prepared chiral mesoporous silicon with C₁₄-L-Iso was used for HPLC to separate racemates,too.