Preparation And Biocompatibility Of Titania Nanotubes/Micron Pit Composite Structure On Titanium Surface

Titanium metal is a hard tissue substitute material with excellent mechanical and biological properties.However,in the period of implantation into the human body,the fibrous tissue layer is generated due to the biological inertness of the surface,which causes the inflammatory reaction to cause implantation failure.Therefore,modification on the surface of titanium has become a widely used method.The micro/nano composite structure can further improve the hydrophilicity of the surface and promote cell adhesion and matrix secretion compared to a single grade?micron or nanoscale?surface.On the one hand,micro/nano grading structures can create a biomimetic environment that is closer to natural bones with micro/nano grading structures.On the other hand,micron structures can also influence and regulate a range of biological processes.Therefore,the preparation of hierarchical coatings has become an important means of surface modification of current biomaterials.In order to improve the biocompatibility of titanium and promote the attachment and differentiation of osteoblasts on its surface,this study designed a novel process for preparing micro/nano hierarchical structures.In order to control the diameter of the experimental nanotubes to prepare a specific tube length of 70⁸0 nm nanotubes,this paper first changed the time,voltage,F^-concentration and other test parameters to prepare TiO₂ nanotubes with controlled diameter.In the process of preparing the hierarchical structure,firstly,micro-scale pits?10²0?m?were prepared on the surface of titanium by the technique of pickling after micro-arc oxidation,and then the oriented growth of titanium dioxide nanometers tubes?70⁸0 nm?was prepared by the anodization technique on the above-mentioned micro-scale pits.Titanium dioxide nanotube/micron pit composite structure was prepared by micro-arc oxidation,pickling and anodizing composite process.The phase composition and hydrophilicity of pure nanotube coatings,micro-arc oxidation coatings prepared with different electrolytes,and composite coatings obtained by different micro-arc oxidation coatings were further studied.Biocompatibility was verified by adhesion and proliferation experiments of human SV40 transfected osteoblasts?hFOB1.19?.The results showed that the composite structure obtained by micro-arc oxidation coating prepared by different electrolytes had significant micro/nano hierarchical structure characteristics.The nanotube diameter was in line with the original design of 70-80 nm,and exhibited excellent hydrophilicity.The results of cell experiments showed that the surface of the micro/nano hierarchical structure deposited hydroxyapatite fast,and it had an advantage in cell adhesion,while the proliferative ability was slightly weaker than the micro-arc oxidation sample.Overall,it had good biocompatibility.