Synthesis Of Functional Nanomaterials And Its Application In ECLIA For Diclofenac,Sudan Ⅰ And Enrofloxacin

Due to wide linear range,high sensitivity,low background noise and good controllability,electrochemiluminescence(ECL)sensing analysis has become a hot spot in the field of analytical methods researching.The researchers highly expect it to be used as a weapon in various scientific fields.Especially after being combined with immunoassay,it can possess high selectivity.That is,it can specifically detect one or a class of substance.This enriches its practical use.At present,the research about ECL immunosensors mainly focus on introducing more suitable nanomaterials to satisfy different requirements.In this paper,a variety of nano-and nano-composites were synthesized and used to construct ECL immunosensors.We aim to improve the performance of ECL immunosensors in different aspects.Several sensors were constructed to detect the harmful small molecule substances added illegally in water and food,such as diclofenac(DCF),Sudan I and enrofloxacin(Enro).At the same time,it also provides a new idea for the detection of other small molecules.This paper mainly includes the following four chapters:In chapter 1,the basic concepts and principles of ECL immunoassay,the role of different nanomaterials in improving ECL immunosensors’ performance and the application of ECL immunosensors in practical samples were introduced.In chapter 2,ECL immunosensors based on Ce:Zno and Pd NPs/PEI-GO/CdSe@CdS QDs for the detection of DCF has been established.These nanomaterials have different benefits for the construction of the immunosensor,and finally can synergistically amplify the ECL signal to achieve ultra-sensitive detection.These materials and the constructed immunosensor were characterized by TEM,SEM,EDS and other tools to insure successful preparation.After that,a standard plot was established under optimal conditions.Final performance testing of the constructed immunosensor shows that it can be used for the detection of DCF in real samples.In chapter 3,ECL immunosensors based on the ECL-resonance energy transfer between Au-g-C3N4 NHs and Au/Cu NFs for the detection of Sudan I has been established.Au-g-C3N4 NHs and Au/Cu NFs act as donor and acceptor,respectively.Through optimizing materials and experimental conditions,excellent quenching effects were achieved.Similarly,nanomaterials and the constructed immunosensor were characterized by a variety of methods to ensure successful preparation.Final performance testing of the constructed immnunosensor indicates that it can be used to detect Sudan I in real samples.In chapter 4,ECL immunosensors based on Au/C60 and Au@BSA for the detection of Enro has been established.For improving the final performance of the immunosensor,many functionalized nanocomposites were introduced to improve its conductivity,load capacity and biocompatibility.These nanocomposites also were characterized by a variety of means to ensure that their morphology and size are suitable for the construction of immunosensor.The performance of the assembled immunosensor was studied and found that the sensor has a satisfactory detection effect for Enro.