Research And Application Of Targeted Bimetal Aunr-pd Nanoparticle In Two Photon Imaging And The Hydrogen Photo-thermal Treatment Of Tumor

Cancer is a leading cause of death worldwide.Traditional monotherapy methods include chemotherapy,gas therapy and photothermal therapy,but they all have their own limitations,so collaborative therapy emerges are required as the times.As a new medical gas,hydrogen has therapeutic effect on many diseases,but hydrogen gas is small molecular size,nonpolarity and low solubility.Therefore,the traditional administration routes have undesirable performance for disease-targeted delivery and controlled release of hydrogen,frequently leading to limited therapeutic efficacy.Nanomaterial provides a promising platform to overcome this challenge.Pd nanocrystal is an excellent hydrogen storage material and ideal catalyst for hydrogenation reaction potentially promoting hydrogen therapy outcomes.Moreover,gold nanorods with excellent NIR thermal effect were combined with palladium to achieve the effect of synergism in tumor treatment.To our surprise,we found that the gold-palladium bimetallic structure not only has excellent two-photon imaging effect,but also is not easy to quench.This hydrogenothermal approach has presented a cancer-selective strategy for synergistic cancer treatment and also has a certain application prospect in imaging.Prepared by seed synthesis method for 40 nm to 10 nm)of gold nanorods(AuNR),according to the principle of complementary base pairing and celeste coordination function,the modification on the uniformity of gold nanorods single-strand DNA(ASY1,ASY2)and Nucleic-acid aptamer(MUC1),and use the oxidation-reduction reaction on the surface structure of the growth of palladium particles,By transmission electron microscopy(TEM),field emission transmission electron microscopy(TEM),nano particle and Zeta potential analyzer,two-photon fluorescence microscopy characterization methods such as the preparation of target of gold palladium AuNR-Pd in the process of double metal nanostructure morphology,elemental composition,particle size and the two-photon fluorescence intensity after cellular internalization.The optimal ratio of the nucleic acid aptomers with targeting function in the structure was ASY2:MUC1=100:1,and it was found unexpectedly that aunr-pd's two-photon fluorescence intensity was stronger than AuNR alone,and it was not easy to quenched.The physical adsorption method AuNR-Pd-H2 preparation nanometer hydrogen absorbing structure,by uv-vis spectrometer,Methylene blue reagent(Methylene blue,MB)test,cytotoxicity test means of characterization,probes into the AuNR-Pd-H2 of the structure of the buffer solution(Phosphate buffer saline,PBS),hydrogen release quantity stability,cell killing ability and best near infrared laser power and cellular internalization.It is worth mentioning that in cell toxicity experiment,the near infrared laser AuNR-Pd-H2 of the structure of the cell to kill your strongest ability,at 25%,Using the photothermal effect of gold nanorods and the hydrogen treatment by palladium crystal adsorption,we observed the killing effect of the structure at the cellular and in vivo level.