Screening And Characterization Of Components In Plants That Improve Glucose And Lipid Metabolism Disorders

Diabetes is a multi-parameter metabolic disease characterized by high blood glucose level.The study of etiology shows that strict control of postprandial blood glucose can prevent or delay the development of diabetes and its related long-term complications.α-glucosidase is a membrane binding enzyme,which exists in the brush border of the small intestine and is responsible for the hydrolysis of oligosaccharides and their decomposition into absorbable monosaccharides.α-glucosidase inhibitor can slow down the hydrolysis process and help to reduce postprandial blood glucose level.Experimental and clinical studies have found that oxidative stress is also a pathogenic factor to promote the occurrence of diabetes and its complications.Potentilla discolor Bge(PDE),Gynostemma pentaphyllum(GPE),Morus alba L(MAE)and Evodia rutaecarpa(ERE),were analyzed for their chemical composition,antioxidative and enzyme inhibition activities.PDE was found to exhibit the highest antioxidant activities in all antioxidant assays.In enzyme inhibition activity test,the inhibition activity of a-glucosidase and tyrosinase of MAE was the highest,both higher than that of positive control,IC50 values were 0.025 mg/mL and 0.008 mg/mL,respectively,and ERE exerted the highest cholinesterase inhibitory activities;the higher the total polyphenol content,the stronger the inhibition ability of xanthine oxygenase.Based on HPLC/Q-TOF-MS,the chemical constituents of PDE and MAE were identified.15 compounds were found in PDE,including quercetin,kaempferol and flavonoid derivatives.Twelve flavonoids and six unknown compounds were found in MAE,including kuwanon g,kuwanon N and sanggenon D.The results showed that PDE may be a potential source of natural antioxidants,and MAE can be used as a natural source of antidiabetic and dermatologic drugs.ERE can be used as the natural plant source of acetylcholinesterase inhibitors and butyryl cholinesterase inhibitors,while xanthine oxidase inhibitors can be selected from plants with high polyphenol content.