The Synthesis And Application Of Functionalized Molybdenum Disulfide Nanocomposites

Molybdenum disulfide(MOS2),one of the graphene-like two-dimensional layered materials,has been widely used in biomedicine due to its unique physical and chemical properties.The specific surface area of MoS2 is high and the surface of MoS2 is easy to modify.Therefore,functionalized nanomaterials were synthesized and applied to detection and as an antibacterial agent.In this paper,we explored the peroxidase-like catalytic activity,catalytic mechanism of MoS2 nanosheets(MoS2 NSs)and established a high sensitivity and good selectivity colorimetric method based on MoS2 NSs with the peroxidase-like catalytic activity for H2O2 and cholesterol detection.Next,MoS2-Lysozyme nanosheets(MoS2-Lys NSs)was obtained with Lys as the exfoliating agent.The antibacterial activity of MoS2-Lys was also explored.The detail was as follows.First,ammonium tetrathiom olybdate((NH4)2MoS4)was used to synthesize MoS2 NSs by one-pot hydrothermal method.MoS2-GSSG NSs were obtained by modifying MoS2 NSs with oxidized glutathione(GSSG).The peroxidase-like catalytic activity,catalytic mechanism of MoS2 NSs were investigated and the steady-state kinetic parameters were calculated.With TMB and H2O2 as substrates,a high sensitivity colorimetric method based on MoS2 NSs with the peroxidase-like catalytic activity for H2O2 and cholesterol detection was established.The result suggested that MoS2 NSs showed high peroxidase-like catalytic activity and the catalytic mechanism was consistent with Ping-Pong mechanism.Meanwhile,the cholesterol in obese mice serum was detected by this method and the level of cholesterol was well agreement with the result detected by blood biochemical method indicating a high sensitivity colorimetric method.The repeatability,reproducibility,and selectivity of this method were also examined.The result suggested a method with good repeatability,reproducibility,and selectivity was established.Next,bulk MoS2 was dealt with concentrated sulfuric acid,then ultrasonicated with Lys obtaining MoS2-Lys NSs with high yield.Lys with positive charge absorbed onto the surface of MoS2 NSs with negative charge due to electrostatic adsorption.Lys as an antibacterial agent could hydrolysis the component of bacterial cell walls,killing bacteria.MoS2-Lys NSs with small size entering the bacterial cell catalyzed H2O2 to ·OH,which had highly effective antibacterial activity.In this paper,MoS2-Lys NSs combining antibacterial agent with peroxidase-like catalytic activity showed highly effective antibacterial activity.The possible antibacterial mechanism was also explored.In this paper,two kinds of MoS2 NSs was synthesized with different modification obtaining functionalized MoS2 nanomaterials.The peroxidase-like catalytic activity and catalytic mechanism of MoS2 were explored.Functionalized MoS2 nanomaterials were applied to detection of H2O2 and cholesterol,and antibacterial.We provided a way for the design and application of functionalized MoS2 nanomaterials in biomedical detection technology and antibacterial area.