Construction Of Woven Scaffold For PLA/PVA/SA Composite Yarn

The tissue engineering scaffold functions as an extracellular matrix(ECM),providing a site for cell adhesion,growth and proliferation,as well as gas exchange.Among them,hydrogel materials have good biocompatibility and three-dimensional microporous structure,and have high application value in biological scaffold materials.A well-functioning hydrogel biomaterial can be obtained by combining a natural polymer with a synthetic polymer.In addition,the preparation of composite scaffolds by combining hydrogels with yarns,fabrics and the like is a hot research topic.The obtained composite scaffolds have the special microporous structure of hydrogels and the excellent controllability and weavability of yarns.The field has potential application value.In this paper,a certain mass ratio of polyvinyl alcohol(PVA)/sodium alginate(SA)hydrogel was combined with polylactic acid(PLA)spun yarn to prepare PLA/PVA/SA composite yarn with good biological properties.The PLA/PVA/SA composite woven fabric was prepared by using PLA/PVA/SA composite yarn as the weft yarn and pure PLA filament as the warp yarn.The surface morphology,chemical structure,mechanical properties,water absorption properties and biological activity in vitro were studied.The specific research contents are as follows.(1)Preparation and properties of PVA/SA hydrogelThe PVA solution and the SA solution were combined,and after freezing,thawing,soaking and drying,a PVA/SA composite hydrogel was obtained.The morphology,elemental composition,group change,water swelling and gel densification were characterized by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),infrared spectroscopy(FTIR),swelling degree and porosity.The results show that the micropore structure of the gel is relatively regular.With the increase of SA content,the gel micropores is changed from dense to loose and the mesh increases.Compared with the pure PVA hydrogel,the swelling degree of the composite hydrogel increased by about 59%,and the porosity increased to 78%.(2)Preparation and properties of PLA/PVA/SA composite yarnThe PLA spun yarn is combined with the PVA/SA hydrogel solution,and after freezing,thawing,soaking and drying,the hydrogel composite yarn is obtained,and further mineralized.The morphology,elemental composition,group change,hydrophilicity and mechanical properties were characterized by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),infrared spectroscopy(FTIR),water absorption and tensile test.The results show that the hydrogel in the composite yarn successfully coats the surface of the yarn and penetrates into the inside of the yarn to form a three-dimensional microporous structure on the surface of the yarn.After mineralization,a large amount of apatite is deposited and grown on the surface of the yarn and the apatite content is more than pure PLA spun yarn,indicating that the composite yarn has good biological activity in vitro;compared with pure PLA spun yarn,the water absorption of the composite yarn is increased by about 50%,and the mechanical properties are enhanced.(3)Preparation and Properties of Woven scaffold for PLA/PVA/SA Composite YarnsThe self-made PLA/PVA/SA composite yarn is used as the weft yarn,and the PLA filament is used as the warp yarn to weaving composite woven scaffold.The plain weave structure and the change satin weave structure are used to weave the composite woven scaffold.And further mineralization.The morphology,elemental composition,group change,water swelling,gel densification and mechanical properties were characterized by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),infrared spectroscopy(FTIR),swelling degree,porosity and tensile test.The results show that the microporous structure of the weft composite yarn in the composite woven scaffold is well preserved;after mineralization,a large amount of apatite is deposited on the surface of the woven scaffold,and the apatite content in the composite woven scaffold is more than pure woven scaffold.And the apatite content of the weft composite yarn is more than that of the warp pure PLA filament,indicating that the composite woven scaffold has good in vitro biological activity;compared with the pure woven scaffold,the swelling degree of the composite woven scaffold increases,and varies slightly with the hydrogel content of the composite yarn.The swelling degree of the satin weave scaffold is about 1.5 times that of the plain weave scaffold;the hydrophilicity of the composite woven scaffold is improved,the porosity is slightly reduced,and the breaking stress in the weft direction is increased.In summary,the PLA/PVA/SA composite yarn,PLA/PVA/SA composite woven scaffold has good in vitro biological activity,water absorption and mechanical properties,and can be used to construct tissue engineering scaffold materials.