Optimization Of Pectinase-assisted Extract Process Of Black Currant Juice And The Preparation Of Its Seed Oil

Black currant is the fruit of Ribes nigrum L.,also known as black currant,black bean and purple berry.It is a common small berry in North China.Black currant fruit contains anthocyanin,polyphenol and other functional components,and has many pharmacological activities such as lowering blood pressure,anti-tumor,etc.The content of ?-linolenic acid in its seeds is higher than that of the recognized plant evening primrose seed oil rich in ?-linolenic acid.Therefore,black currant fruit has high nutritional value,medicinal value and economic value.In this paper,blackcurrant fruit was studied from two aspects:pectinase assisted extraction of blackcurrant fruit juice and enzyme assisted extraction of seed oil.The main contents are as follows:1.The extraction of black currant fruit juice was carried out by pectinase assisted extraction method.Through single factor experiment and response surface analysis experiment,the extraction process of black currant fruit juice was studied.The optimal extraction process parameters were:pH 2.67,enzyme addition 0.08 g/L,enzyme hydrolysis temperature 50?,and enzyme hydrolysis time 2.2 h.Under this condition,the extraction rate of juice was 61.19%,and the content of polyphenols was 6457.89 mg/L.2.Principal component analysis and two-dimensional scatter diagram were used to analyze the correlation between the pectinase processing parameters and the quality of black currant juice.For the extraction rate of fruit juice,when pH value is 3-5,enzyme treatment time is 2-3 h,enzyme addition is 0.06-0.08%,and enzyme treatment temperature is 40-50?,higher extraction rate can be obtained;for the pectin content of fruit juice,when pH value is greater than 5,pectin content is higher,and the lower pH value is,the lower pectin content is,and enzyme treatment time is 1-2 For the soluble dietary fiber content in fruit juice,the enzyme treatment time was 1-2 h,and the enzyme addition was 0.04-0.08%For the soluble solid content of blackcurrant juice,when the pH value is 2?4 and the enzymolysis time is 1.5?2.5 h,more soluble solid can be obtained.This results are consistent with the response surface analysis.3.The method of enzyme assisted organic extraction was used to extract blackcurrant seed oil.The extraction rate of the seed oil treated by cellulase was 25.0%higher than that of the seed treated by enzyme.The optimum conditions of seed oil extraction were pH=5,enzyme reaction time 60 min,enzyme hydrolysis temperature 50?,average extraction rate of seed oil 18.0%.Through the above experimental research,we can make full use of blackcurrant fruit,and get high-quality blackcurrant juice and rich in y-linolenic acid seed oil under the optimal technological conditions.The results of this paper can provide theoretical guidance for the deep processing of blackcurrant fruit and the development of high-value products(commodities).