Study On Synthesis Of Basic Materials Of Nas/dma Copolymer Element Tag Framework With New Porphyrin Rings As Side Groups

Inductively coupled plasma mass spectrometry(ICP-MS)is a sensitive element analyzer based on mass spectrometry.In recent years,ICP-MS technology has been more and more applied to the field of biological analysis,by measuring the content of biomolecules Specific elements to determine information about biomolecules.At present,the immunoassay method based on the element-labeled ICP-MS technology has been widely used in the quantitative analysis of various biomolecules.Element tagging is the most important basis for the quantitative analysis of biomolecules by ICP-MS technology.Its composition includes three parts:chemical groups that can be attached to the target biomolecules,polydentate ligands chelated to the metal,and metal ions.In the application process of element tag-based ICP-MS technology,the sensitivity of its analysis is related to the number of metal ions contained in the element tag.The metal polymer uses a polymer as the skeleton of the element tag and combines multiple polydentate through active functional groups.Ligand,which can increase the number of metal ions on the element label.In this paper,a random copolymer Poly(DMA-co-NAS)was synthesized by RAFT polymerization method using N-acryloyloxysuccinimide-NAS and N,N-dimethylacrylamide-DMA.The synthesized copolymer was characterized by gel permeation chromatography(GPC)combined with nuclear magnetic resonance hydrogen spectroscopy(1H-NMR),and it was analyzed that a random polymer with a narrow molecular weight distribution was synthesized in this experiment.Guided by the orthogonal experiment method,several groups of experiments were scientifically designed to investigate the effects of polymerization reaction conditions(reaction temperature,reaction time,ratio of chain transfer agent to initiator,amount of chain transfer agent)on the polymerization effect,and this polymerization reaction was obtained.The influence of the reaction conditions and the optimal experimental conditions for the polymerization reaction were optimized.That is,at a reaction temperature of 85?,the ratio of chain transfer agent to initiator is 5,the amount of chain transfer agent is 0.158 mmol,and the polymerization can be obtained after 20 hours of polymerization Ideal random copolymer with narrow molecular weight distribution and large molecular weight.And through the analysis of experimental influencing factors,the influence of experimental conditions on the polymerization reaction is obtained,that is,polymers with higher molecular weight and narrow molecular weight distribution can be obtained at high temperature,high chain transfer agent/initiator ratio,and longer reaction time.It is very meaningful to better select the reaction conditions for this type of polymerization.Using 5-(4-aminophenyl)-10,15,20-triphenylporphyrin to chemically react with the functional group of the NAS unit in the above copolymer,and confirmed this connection by nuclear magnetic resonance hydrogen spectroscopy Real existence.The new polymer material designed and synthesized can be used in the element label introduced above.As the skeleton material of the element label,the presence of the porphyrin ring can enable it to perform metal complexation in subsequent applications.