The Study On Preparation And Properties Of Antibacterial And Anti-adhesion Gauze-Lotus@Gauze

This is not only because gauze is cost-effective and easy to use,but also because they can be physically isolated to protect the skin from bacterial infections of the wound.However,the use of gauze may worsen the regeneration of the wound due to the following two reasons:1)Gauze can cause serious infections because the wound expansion is absorbed into the gauze,which provides a proper moisturizing and nutrient environment for the growth of bacteria 2)As the wound enlargement penetrates into the gauze,the gauze will stick tightly to the injured skin and damage the newly formed skin when changing the dressing,resulting in"secondary injury".Wound infections with more secondary injuries(caused by daily or routine dressing changes)can significantly prolong the process of wound healing,which can lead to serious complications,especially for patients with underlying diseases such as diabetes.To solve the above two problems,gauze is highly expected to have anti-blocking and antibacterial properties.However,very few mesh yarns can achieve both functions at the same time.Therefore,there is an urgent need for development.Based on the above research points,the research content of this article is mainly divided into the following parts:1.We developed anti-blocking and antibacterial medical gauze with lotus leaf simulation in just three simple dip-coating steps.First,the ordinary gauze was dipped into a dopamine trimethylolamino buffer solution to perform PDA coating.Then,based on the reaction of the PDA and cholesterol based-perfluorocarbon,the gauze was immersed in a perfluorocarbon solution to form a hydrophobic layer.Finally,the gauze was immersed in a silver nitrate solution to germinate Ag NPs in situ to obtain the expected product(EDS),water contact angle measuring instrument and other detection methods,characterizing the successful synthesis of Lotus@Gauze gauze.2.In fact,700-1100 nm infrared(NIR)has deeper penetration of tissues,which translates into biological applications.Similarly,near-infrared-triggered PTT usually requires a temperature of 50℃or higher to induce protein destruction and,in addition,alternative temperature(eg,℃)strategies cannot achieve higher antibacterial effects in a short period of time.Polydopamine(PDA)provides a new idea for enhancing photothermal properties,because it has a similar molecular structure as the orientation protein of mussels.The catechol group of PDA can perform hydrogen bonding,electrostatic interaction andπ-πinteraction with various surfaces,so that not only does the material have good cell/tissue adhesion properties,but also because of its degradability It has excellent biocompatibility without any unexpected adverse effects in the body.Because it contains PDA,the photothermal effect temperature induced in the near infrared(NIR)can be increased by nearly20℃,which enhances the antibacterial effect of gauze.3.The antibacterial properties of Lotus@Gauze come from Ag NPs,the core of which is Ag~+released by silver nanoparticles to destroy the cell wall of microorganisms.Later,through a series of antibacterial experiments,including inhibition zone experiments,optical detection,bacterial co-culture and so on,it was confirmed that the Lotus@Gauze gauze had good biocompatibility.The cell death staining and toxicity experiments confirmed that the lotus@Gauze has good biocompatibility and cell safety.The lotus@Gauze has a slow release time and high release efficiency,which is more conducive to the application of later wound healing.4.The infection wound model of C57BL/6 mouse full-thickness skin resection was used to evaluate the wound healing efficiency.The antibacterial infection can cause severe tissue damage,so we use Staphylococcus aureus to rapidly infect wounds to mimic wound infection.In vivo experiments have shown that Lotus@Gauze can significantly reduce wound residues,effectively accelerate wound healing in mice,promote granulation tissue proliferation,and can promote local skin deposition and distribution in wounds.NIR also has the effect of fast wound healing.The laser and Lotus@Gauze can easily interact with each other,which can further effectively accelerate the wound healing speed.