Effects Of Probiotics On Gastrointestinal Microbiota,Fatty Acid Metabolism And Meat Quality Of Sunit Sheep

24 Sunit sheep of 3 months old were randomly divided into two groups in this trial,which were the control group?basic diet?and the probiotic group?basic diet+1.50*109CFU/g compound probiotics?,After 90 days of feeding,the longissimus dorsi muscle,blood,rumen fluid and intestinal feces were taken as test materials,The differences of fatty acid composition and gastrointestinal microflora structure were studied by GC-MS and high-throughput sequencing.It is known that the sheep's weight gain and eye muscle area,the a*value,intramuscular fat,ash and the amino acid content of the lambs in the probiotics group were significantly higher than those of the control group?P<0.05?,the pH value,b*value and shear force value were significantly lower than the control group?P<0.05??It is known that the content of SFA and MUFA in the control group was higher than that in the probiotic group?P<0.05?,while the content of PUFA was significantly lower than that in the probiotic group?P<0.05?.The contents of HDL and ALB in the blood of the probiotic group were significantly higher than those of the control group?P<0.05?,while the contents of LDL and BUN were significantly lower than those in the control group?P<0.05?.The a-diversity of rumen and gut microbiome in probiotic group was higher than that in control group?P<0.05?.At rumen microbiome phylum level,The relative abundances of Bacteroides,Actinomycetes and Verrucomicrobia in probiotics group were significantly higher than those in control group?P<0.05?,while Firmicutes and Proteobacteria were significantly lower than those in control group?P<0.05?;At the genus level,the relative abundance of prevotella-1,Bacteroides,Saccharofermentans and Fibrobacter in probiotics group was significantly higher than that in control group?P<0.05?.At gut microbiome genus level,the relative abundance of Bacteroides,Christensenellaceae_R-7_group,Ruminococcaceae_UCG₀02,unclassified_fLachno spiraceae in probiotics group was significantly higher than that in the control group?P<0.05?.By analyzing the correlation between the gastrointestinal flora,meat quality and fatty acid content.Rumenococcus-1 in the rumen flora was significantly negatively correlated with a*and significantly positively correlated with shear force?P<0.05?;In the intestinal flora,Ruminococcaceae_UCG₀02 showed a significant negative correlation with shear force,and Succinivibrio was significantly negatively correlated with cooking loss?P<0.05?;An increase in Bacteroides in the rumen flora favors alpha-linolenic acid deposition.Among the intestinal flora,Ruminococcaceae_UCG₀02,[Eubacterium]_coprostanoligenes_group were significantly positively related to PUFA?P<0.05?,Phascolarctobacterium,Akkermansia were significantly positively correlated with EPA deposition?P<0.05?.