Application Of FI-CLIAs Combined With Enzyme Amplification Strategies In Environmental And Food Safety

With the continuous improvement of people's living standards and consumption concepts,"Green and Health" has gradually become synonymous for the pursuit of spiritual substances by the general public.Meanwhile,food and environmental safety issues have aroused heated discussion among citizens.In order to detect environmental pollutants and food banned additives sensitively and accurately,it is the general trend to develop an efficient analysis and detection method.Flow injection chemiluminescence analysis is a rapid response,simple operation and high sensitivity detection method,which has been widely used in medicine,food,environment,pesticides and other fields closely related to life.In order to improve the specificity of the analysis and detection method,it is combined with immunoassay based on antibody-antigen reaction,and flow injection chemiluminescence immunoassay is constructed using the advantages of the two methods,which greatly widens the practical application value of the two methods.On this basis,this paper carries out a series of signal amplification strategies for the detection of food banned additives and environmental pollutants.Several sensors constructed have been successfully used to detect water samples,such as mercury ion and small molecular harmful substances such as bromobutrol,chloramphenicol added in the field of food safety,providing an effective analysis method for the research and application of food safety and environmental pollution.This paper mainly includes the following aspects:1)Carboxylic resin beads are used as solid phase carriers to connect antigens,because of their large specific surface area and good biocompatibility,they can load a large number of antigens.The introduction of high affinity polyclonal antibody and horseradish peroxidase labeled secondary antibody can effectively amplify chemiluminescent signals.The linear range of this method for the detection of brombuterol is 0.001-300 ng mL-1.A flow injection chemiluminescence immunosensor with wide linear range,simple sample preparation and high sensitivity was established to detect bromobutrol residues in pork meat and feed.2)Silica nanoparticles with good dispersibility and uniform particle size were prepared and successfully modified chemical group on the surface.Silica nanoparticles modified with antibody and horseradish peroxidase were synthesized as immune probes,which greatly increased the load of enzyme.Based on the signal amplification effect of horseradish peroxidase on luminol-p-iodophenol-hydrogen peroxide system,the immunosensor has good reproducibility,fast response speed and low detection limit for the determination of chloramphenicol,the linear range of response to chloramphenicol is 0.0001-100 ng mL-1,and has been successfully applied to the determination of chloramphenicol in shrimp and honey samples.3)A novel flow cell modified by graphene oxide and chitosan was proposed for flow injection chemiluminescence detection of mercury ions in water samples.Graphene oxide-chitosan composite film provides a good microenvironment for the chemiluminescence reaction of luminol-p-iodophenol-hydrogen peroxide by immobilizing coated antigen and introducing horseradish peroxidase labeled secondary antibody,which can effectively enhance the chemiluminescence intensity.The new self-made immunosensor has the advantages of fast detection,high specificity and wide linear range,and has achieved good results in the detection of Hg2+ in actual water samples.The linear range of Hg2+ detection is 0.001-300 ng mL-1.The successful development of this method can also be used to detect other environmental pollutants,expanding the practical application range of flow injection chemiluminescence immunoassay.