Practical Research On Synergistic High-yield DHA/EPA For Microalgae Treatment Of Special Wastewater

High-salt glycerin wastewater widely exists in industries such as epichlorohydrin production and biomass refining.Due to its high salt content,it is difficult to biochemically treat it.In addition,the pharmaceutical industry and livestock and poultry farming and other industries produce a large amount of antibiotic wastewater,which not only induces the generation of resistance genes,endangers human health,but is also difficult to degrade due to poor biochemical properties.In order to effectively treat these two special wastewater,the study found that marine microalgae can be well adapted to the environment of high-saline wastewater through domestication,and some microalgae can also degrade specific antibiotics,and use the nutrients in the wastewater through microalgae Produce high value-added resources,including polyunsaturated fatty acids,with extremely high medical and nutritional value;in addition,ordinary fatty acid methyl ester can be used as biodiesel.In this paper,Tisochrysis lutea and Porphyridium cruentum are used to treat two kinds of artificial wastewater.The specific experiments and conclusions are as follows:By setting seven different light qualities,the effect of light quality on the growth of Tisochrysis lutea was investigated.According to the experimental results,Tisochrysis lutea grows best under white light irradiation.Secondly,under the irradiation of red light and green light,the growth condition is better.By setting different salinities,the growth of Tisochrysis lutea and the absorption of glycerin were investigated.The increase of salinity can damage the growth of Tisochrysis lutea,and the presence of glycerin will also promote the growth of Tisochrysis lutea.At the end of the 35-day experiment.The increase of salinity will also limit the absorption and degradation of Tisochrysis lutea to nitrogen,and the presence of glycerol can promote the absorption of nitrogen by Tisochrysis lutea.The increase of salinity will slow down the absorption of phosphorus by Tisochrysis lutea,but for the initial concentration of 10 mg/L phosphorus,the degradation rate can reach about 97.99%.Tisochrysis lutea can degrade a certain amount of glycerin.Six groups of nitrogen and phosphorus concentration conditions were set to observe the growth of Tisochrysis lutea,the absorption of glycerol and the production of fatty acid.Under the condition of sufficient nitrogen and phosphorus,Tisochrysis lutea grows best,and the biomass is the highest at the end of the experiment.It is found that the lack of nitrogen will seriously limit The growth of Tisochrysis lutea.In the case of sufficient phosphorus,Tisochrysis lutea has the best degradation effect on nitrogen,and the decrease of phosphorus concentration will reduce the degradation of nitrogen by Tisochrysis lutea.The presence of nitrogen will promote the absorption and degradation of phosphorus by Tahitian gold algae.Under sufficient nitrogen and phosphorus conditions,Tisochrysis lutea has the best degradation effect on glycerol.Under the conditions of sufficient nitrogen and phosphorus limitation,the THA produced the highest concentration of DHA,EPA and TFA.Using microalgae treat chlortetracycline wastewater.Two kinds of microalg ae were selected,with algae and bacteria group,non-algae and bacteria group a nd non-algae aseptic group,respectively,to investigate the treatment effect of c hlortetracycline.The result show the Tisochrysis lutea cannot degrade chlortetra cycline.Porphyridium cruentum can grow in chlortetracycline wastewater,and ca n degrade chlortetracycline hydrochloride in wastewater,and the degradation rat e of bacteria and algae group can reach 78%,and the degradation effect is ob vious.