Study On Detection Method Of Synthetic Pigment Based On Three-dimensional Fluorescence Technology

With the development of food industry,a variety of food additives are produced.Colorful beverages are also the result of adding various pigments to beverages.Pigments can be divided into natural pigments and synthetic pigments.Natural pigments are mainly from plants and are naturally harmless.When the concentration of synthetic pigments exceeds the specified standard,it will endanger human health.Therefore,the determination of pigment concentration in beverages is necessary.In order to avoid harm to human body caused by excessive pigment concentration,it is of great significance to study accurate,rapid and sensitive detection methods of pigment in beverages.There are many methods for the detection of pigment,among which fluorescence detection technology has some advantages due to its high sensitivity and trace detection,so three-dimensional fluorescence spectrum technology is selected for the detection of pigment.Firstly,it introduces the principle and characteristics of fluorescence,analyzes the factors that affect the fluorescence intensity,expounds the relationship between the fluorescence intensity of the tested substance and its concentration,and analyzes the feasibility of detecting the edible synthetic pigment by fluorescence method.The synthetic pigment carmine and amaranth are isomers of each other,and the fluorescence spectrum overlaps seriously,so they are representative.The two pigments are selected as the research object to prepare different concentration single component solutions and different concentration proportion mixed solutions.Secondly,the FLS920 steady-state fluorescence spectrometer is used to scan all samples to obtain the corresponding original three-dimensional fluorescence spectrum data;the original spectrum is used to remove the Raman scattering by subtracting the blank method,and the Delaunay interpolation method is used to process the Rayleigh scattering band.In view of the redundant noise in the obtained fluorescence spectrum,the wavelet threshold algorithm is used to denoise the fluorescence spectrum data.Then,for the general second-order correction method needs to estimate the component fraction in advance,using the alternating residual trilinear method to analyze the sample data of different concentration proportion of carmine and amaranth.When the analysis results are similar to the general second-order correction method,it is more convenient to use.Finally,the spectral data are analyzed by the fast independent component analysis algorithm,and compared with the results obtained by the alternative residual trilinear method,the difference between the concentration prediction results is small,but the analysis speed is better than the alternative residual trilinear method.The recoveries of the two methods were 98.54%~105.8%,and the root mean square error of the two methods was less than 0.018 ?g/ml.