Research On Determination Technology Of Quinolones Residues By Three-Dimensional Fluorescence Spectroscopy

In recent years,the problem of antibiotic residues has been widely concerned.The flow of breeding wastewater and medical wastewater through rivers form antibiotic deposition will have a bad impact on ecological health,and domestic water will also be polluted.In addition,improper use of antibiotics during breeding will cause residual antibiotics in animal source food and threaten people's health.Therefore,it is of great significance for environmental protection and human health to establish a rapid and accurate method for the detection of antibiotic residues.In this paper,the three-dimensional fluorescence spectrum technology combined with the second-order correction algorithm is used to detect quinolones residues in the water environment and honey.It not only solves the common spectral overlapping problem in the complex system,but also realizes the "chemical separation" to "mathematical separation",achieving rapid and accurate detection.The main research work of this paper are as follows:(1)The luminescent principle,luminescent conditions and influencing factors of fluorescence are studied,and the feasibility of fluorometry for quinolones is confirmed.FS920 fluorescence spectrometer is used to detect the spectra of three quinolones,namely flumequine,enrofloxacin and levofloxacin.(2)In order to solve the noise interference in the spectrum,Wavelet,Ensemble Empirical Mode Decomposition(EEMD)and Wavelet Optimization EEMD are studied to denoise the spectrum,which paves the way for the subsequent analysis work.(3)In order to solve the problem of quinolones residues in water,three samples of quinolones in water were prepared.Two kinds of second-order correction algorithms,parallel factor analysis(PARAFAC)and bilinear least squares / residual bilinearization,BLLS/RBL),have realized the qualitative and quantitative of different quinolones mixed component.The comparison of the evaluation indexes of the detection results of the two methods proves that BLLS/RBL algorithm is more suitable for the he detection of quinolones.(4)In order to solve the problem of quinolones residues in honey,three kinds of quinolones in honey were prepared,which can further verify that three-dimensional fluorescence spectroscopy combined with BLLS/RBL algorithm can still accurately detect the mixed components in the complex background of honey.