Preparation Of Polypyridyl Ruthenium(Ⅱ) Complexes And Their Analytical Applications

Ruthenium（Ⅱ）complexes with polypyridine have good photostability,large Stoke shift,high luminescence quantum yield,long phosphorescence lifetime and good biocompatibility,which have been widely used in optical sensors,light emitting devices,dyes sensitive solar cells and photocatalysis.Due to low detection limit,strong specificity,simple operation and availability for biological imaging,luminescent（fluorescence,phosphorescence,electrochemiluminescence）probes showed important application value among various detection methods,In the research of this thesis,based on the polypyridyl ruthenium complex（Ⅱ）phosphorescent probe with specific responses to molecules and ions were synthesized.1.Ru-CIP,a polypyridyl ruthenium complex was synthesized by using4-carboxy-imidazo[4,5-f][1,10]-phenanthroline（CIP）as ligand.The micellar environment created by SDBS adjusts the luminescent properties of Ru-CIP and enhanced the luminous intensity significantly.So a novel phosphorescent probe could be assembled by SDBS and Ru-CIP.Since the absorption spectrum of dopamine overlaps with the emission spectrum of Ru-CIP in the SDBS medium,DA as energy acceptor,Ru-CIP-SDBS assembly as energy donor,that Forster resonance energy transfer（FRET）is a way to quench the luminescence.The method has the advantages of high sensitivity,good selectivity,operation in a wide linear range,easy to operate,fast response,secure and low-cost,etc.It is suitable for the specific detection of dopamine in aqueous solution.2.The synthesized Ru-CIP has a strong characteristic photoluminescence when it is excited at 460 nm.And a huge quenching phenomenon of the luminescence of Ru-CIP was observed when Sudan I is present in ethanol solution based on further work.In the quenching range of0.8-100μM,the luminous intensity of Ru-CIP showed a good linear relationship with the concentration of Sudan I.The detection limit was 0.26μM.It was successfully applied to the detection of Sudan I in chili powder samples.3.RuL,a kind of polypyridyl ruthenium（Ⅱ）complex with a specific response to Cu²⁺was synthesized.None interferential detection can be achieved even in the presence of cations,anions and amino acids,because the chelation effect between Cu2+and RuL could result in the quenching phosphorescence of the ruthenium complex at 621 nm.The luminous intensity of the complex has a good linear relationship with the concentration of Cu²⁺in the range of0.25-12μM,and the detection limit is as low as 83.3 nM.It was successfully used in the detection of Cu²⁺in enviro nmental water samples and domestic water of Taihu Lake in China.It can be further used for the imaging detection of Cu²⁺in living cells A549 by connecting with Laser confocal technology.4.The electrochemiluminescence properties was further studied by using electrochemical methods based on the chelation of Cu2+with the complex RuL,and it was found that the RuL-TPrA（tripropylamine）system has selective recognition to Cu²⁺in solution,which could resulted in the quenching of the electrochemiluminescence（ECL）signal.The measured ECL signal intensity of RuL-TPrA showed a good linear relationship with the Cu2+concentration range of 0.047-9.5μL,and the detection limit（LOD）was15.6 nM.