Studies On Synthesis And Properties Of Ruthenium Complexes-based Probes For Metal Ions

Transition metal ions play an important role in the fields of biology,industry,and environment.How to characterize and detect metal ions has attracted s attracted widely attention.Copper ions(Cu²⁺)and iron ions(Fe³⁺)are two of the most important transition metal ions in humans and animals.Among metal ions,copper ranks the third place in abundance of human trace elements.Cu²⁺,as a component of proteins and enzymes,is an essential trace element in the metabolic process.Normally,the copper content in the human body is about 100?120mg.Trace amounts of copper not only provide the energy for physiological processes,but also promote the formation of heme in the blood.However,excessive copper ions accumulate in the human body,which will cause diseases and cause environmental pollution.Similarly,as one of the essential trace elements,iron plays an crucial role in osmotic regulation,catalysis,metabolism,biomineralization and signal transduction.However,excessive intake of Fe³⁺can lead to a variety of diseases,such as Alzheimer's disease,Huntington's and Parkinson's diseases,as well as some environmental problems.Therefore,it is of great significance for biological research and environmental science to develop a method to detect Cu²⁺and Fe³⁺with high selectivity and sensitivity.Many efforts have been made in the development of metal ion detection in the past few decades,including atomic absorption,voltammetry,inductively coupled plasma atomic emission spectrometry,and chromatography.Although these methods can detect metal ions with relatively ideal sensitivity and detection limits,they have some disadvantages that limit their further widespread use.For example,the detection of metal ions depending on the above methods requires Large equipment,high cost,professional operation and long analysis time.Because of its excellent performance of low cost,high sensitivity,high selectivity and fast response,fluorescence method is widely liked by people.At the same time,colorimetry method is widely used because it is convenient,fast,and can even achieve the purpose of detecting the concentration of ions in the naked eye.In this paper,two novel ruthenium complex are designed and synthesized.These complexes adopt two different coordination modes,one can be used as a colorimetric recognition probe and the other can be used as a fluorescent recognition probe.The selectivity,sensitivity and complexation mechanism of two probes to metal ions have been studied systematically.In addition,we explored their applications in test strips experiment and water samples.In the first chapter,we discussed the significance of Cu²⁺and Fe³⁺,the detection methods of common metal ions,the research status of ruthenium complexes and the research progress of Cu²⁺and Fe³⁺.At last,the main content of the research topic of this thesis is put forward.In the second chapter,we prepared a ruthenium complex with Schiff base,which can simultaneously be used as a"naked eye"probe to detect Cu²⁺and Fe³⁺in acetonitrile solution and test paper at the same time.the limit of detection?LOD?for detection of Cu²⁺can be as low as 3.26×10^-8M,with visible color change from pink to colorless.the limit of detection?LOD?for detection of Fe³⁺is 3.12×10^-7M,and the color of solution changes from pink to light yellow.At last,we synthesized a fluorescent probe based on ruthenium complex,which can specifically recognize Cu²⁺from other metal ions.The probe possesses long excitation wavelength,high selectivity and sensitivity for detection of Cu²⁺,and the limit of detection?LOD?for detection of Cu²⁺is 1.78×10^-8M.In addition,the probe has been successfully used in the paper strip experiment and detecting Cu²⁺in environmental real water samples with excellent recoveries.