Development And Application Of High-Efficiency Bactericide In Tobacco Rods

In this study,21 strains of cellulose-degrading bacteria were screened out from tobacco straw waste through enrichment culture and streak separation.The high-efficiency cellulose was re-screened through Congo red cellulose culture and CMCase and FPase enzyme activity detection.4 strains(SL-2A,SL-3A,SL-7 and SL-12).Morphological observation and molecular biological identification of the strain showed that: SL-2A was Bacillus aerophilus,SL-3A was Bacillus subtilis,SL-7was Bacillus amyloliquefaciens,and SL-12 was Bacillus altitudinis.The fermentation results of the four cellulose-degrading bacteria strains showed that the four strains CMCase and FPase reached the highest on the third day,SL-2A was 48.72 U / mL and33.61 U / mL,SL-3A was 96.98 U / mL and 70.43 U / mL,SL-7 is 84.81 U / mL and37.38 U / mL,SL-12 is 52.61 U / mL and 24.33 U / mL.Optimize the enzyme-producing culture conditions of the four strains obtained from the re-screening,including the effects of inoculation volume,initial pH,temperature,and temperature and rotation speed on the enzyme production of the strain;meanwhile,the components of the culture medium were optimized,including nitrogen and carbon sources.And carbon-nitrogen ratio on the enzyme production of the strain.After the optimization of the enzyme-producing culture conditions,the CMCase and FPase of strain SL-2A increased by 50.31% and 69.52% respectively before optimization;the CMCase and FPase of strain SL-3A increased by 12.70% and49.46% respectively before optimization;The CMCase and FPase of SL-7 were increased by 46.87% and 54.32% respectively before optimization;the CMCase and FPase of strain SL-12 were increased by 112.25% and 51.61% respectively before optimization.After optimizing the medium composition,the CMCase of strain SL-2A was improved by 35.43% compared to the optimization;the CMCase of strain SL-3Awas increased by 16.22% compared with that before optimization;the CMCase of strain SL-7 was increased by 18.29% compared with before optimization;The CMCase of SL-12 was increased by 42.67% compared with that before optimization.The microbial screening agent ZZ selected and constructed by the laboratory from tobacco straw waste with high-efficiency cellulose degradation ability was selected,and the biological organic fertilizer(EM bacterial liquid)WG was purchased as different bactericides for different treatments of tobacco straw.The treatment without inoculating the inoculum KB was used as a control group for composting of tobacco straw,and the effect of the inoculation selected was explored.Throughout the composting process,changes in odor and color,temperature,moisture content,pH,conductivity,crude cellulose,total nitrogen,total carbon,crude ash,and humus were measured,and tobacco was germinated by germination tests on cucumber seeds.Toxicity and maturity of straw compost were evaluated.Comprehensive compost detection maturity indicators showed that the addition of homemade bacterial agent ZZ could promote the fermentation of tobacco straw compost,shorten the maturation time,and effectively improve the compost quality.Among them,the bactericide-promoting effect: ZZ bacteria> WG bacteria> CK.Using three different bio-organic fertilizers stacked in this experiment,a corn pot experiment was conducted.The actual effect of compost was monitored by monitoring the plant height,root length,leaf length,leaf width,total weight,stem weight and root weight of the corn plant.to evaluate.It was found that the tobacco straw compost treated by the ZZ fungicide had a certain growth promotion function,and the actual effect on the growth of corn showed that: ZZ fungicide> WG fungicide> CK.