Preparation And Preliminary Application Of Molecularly Imprinted Template Of Ginkgolide B

Ginkgolide B(GB)is a biologically active ingredient in Ginkgo biloba leaves,and is the strongest PAF antagonist found in natural products to date.There are many methods to obtain GB.Although there are many ways to obtain GB,in view of the problems of time-consuming and poor selectivity of traditional methods,with the advantage of molecularly imprinted polymers(ie imprinted template,MIPs)in separation and enrichment,we prepared MIPs to achieve GB rapid separation of components.In this study,GB was used as the template molecule.First,functional monomers were simulated and screened,and GB molecularly imprinted template(GBMIPs)was prepared by traditional methods.Secondly,GBMIPs was loaded on the surface of 3D reduced graphene oxide(3D RGO)aerogel loaded on the surface of 3D reduced graphene oxide(3D RGO)aerogel to obtain 3D RGO molecularly imprinting template(3D RGO-GBMIPs),and the 3D RGO-GBMIPs was characterized and evaluated.Two molecularly imprinted templates were applied to the separation of GB from real samples.Main conclusions are mentioned as follows.(1)The best functional monomers were selected,the traditional preparation process of GBMIPs was optimized,and its performances were investigated.Through computer simulation and experiments,the best functional monomer and the better preparation process were defined.At the same time,combined with Fourier transform infrared spectroscopy(FTIR),field emission scanning electron microscopy(SEM),surface area and porosity analyzer and other characterization means to determine whether the GBMIPs is successfully prepared.The results showed that the adsorption equilibrium concentration was 0.7 mg/m L,and the adsorption equilibrium time was 4 h.It had good selectivity and good reusability for GB.(2)The new adsorbent 3D RGO-GBMIPs was prepared,its structure and adsorption mechanism were studied.On the basis of the previous chapter,the new adsorbent 3D RGO-GBMIPs was prepared by surface molecularly imprinting technique.Various characterization results showed that GBMIPs was successfully loaded on the 3D RGO surface,and the thermal stability was also improved.The adsorption experiments showed that 3D RGO-GBMIPs had a high adsorption capacity for GB and a short equilibrium time.It was in accordance with the pseudo-second-order kinetics model and Freundlich isotherm adsorption model.The selectivity experiment showed that the adsorption selectivity of 3D RGO-GBMIPs to GB is better than that of GA and GC.(3)The dispersive solid phase extraction(DSPE)experiments results of two molecularly imprinted templates in real samples were compared.The 3D RGO-GBMIPs obtained from the experiment were optimized for DSPE.Then,the application effects of GBMIPs and 3D RGO-GBMIPs in practical samples were compared.It was found that the effects of two molecularly imprinted templates on the separation and purification of Ginkgo biloba extract were better than that in 80% purity GB samples,and the effect of 3D RGO-GBMIPs on the separation of GB was significantly better than that of GBMIPs.