Study On The Enrichment And Biodegradation Of Estrogen Contaminants In Aquatic Products

This study established a method for the efficient extraction of estrogen from aquatic products.High-performance liquid chromatography?HPLC?method was used to analyze commercially common commercial aquatic products such as Ruditapes philippinarum,Sinonovacula constricta,Busycon couldaliculatu,Nassarlidae,Larimichthys,Macrobrachium nipponense,Eriocheir sinensis.Detection and qualitative analysis were performed by gas chromatography-tandem mass spectrometry?GC-MS?to study characteristics of enrichment of aquatic products to estrogen pollutants.In order to solve the fundamental problem of the accumulation of estrogen pollutants in water,we had launch the biodegradation of estrogen pollutants.We compared the differences in the degradation ability of different strains with others,discussed the optimal biodegradation conditions.The findings were as follows:1?We optimized the extraction conditions of estrogen in aquatic products,and established an method of HPLC for estrogen detection.We analyzed the effects of different extraction solvents,different extraction times and different extraction solvent volumes on the extraction efficiency of hormones in aquatic products.We established the best extraction method for estrogen in aquatic products:the best extraction agent was chloroform and the extraction time was 20 min,the extraction rate of hormones in aquatic products could reach more than 95%.The results of HPLC analysis showed the optimal chromatographic conditions were:C18column,mobile phase:acetonitrile:water=1:1?volume ratio?,detection wavelength was 225nm,flow rate was 1 m L/min,and column temperature was 25?.The injection volume was 5?L.2?HPLC test of estrogen in aquatic products.Several commercially aquatic products:Ruditapes philippinarum,Sinonovacula constricta,Busycon couldaliculatu,Nassarlidae,Larimichthys,Macrobrachium nipponense,Eriocheir sinensis were tested by HPLC.The results showed different aquatic products had different levels and locations of estrogen enrichment.Estrogen pollutants are mainly concentrated in Busycon couldaliculatu and Nassarlidae,Larimichthys with levels of 24.7 ng/g and 14.2 ng/g,respectively.3?Screening of hormone-degrading strains.The selected nine natural steroid degrading strains were tested for estrogen degradation in contaminated water,the results showed:The degradation efficiency of Comamonas testosterone?CT1 and KF-1?was the higher.CT1 showed the stronger degradation capacity for estradiol.The degradation rate was 48.5%when cultured at27?and 180 rpm for 20 days.KF-1 had the stronger degradation capacity for ethinyl estradiol,and the degradation rate was 21.3%when cultured under the best conditions for 20 days.CT1and KF-1 were mixed to make a mixed flora.The degradation efficiency of estrogen is higher than that of single bacteria,and the degradation rate is increased by 20.6%and 14.6%respectively.4?Effects of p H and metal ions on estrogen biodegradation.At p H was 8.5,the microbial flora had a strong ability to degrade estradiol,which was 42.3%,and the ability to degrade ethinyl estradiol was 37.4%at p H was 5.0.In addition,Cu²⁺and Mn²⁺could promote the degradation of estradiol,whether single or mixed.Among them,both copper ions and manganese ions can promote the degradation of estradiol.Among them,Cu²⁺can greatly improve the degradation efficiency,and the degradation can be increased by 30.0%?63.0%when cultured to 10 days;and Mn²⁺can improve the degradation ability of ethinyl estradiol,which can be increased by 2.0%?15.0%when cultured to 10 days.This study explored the enrichment of estrogen in different aquatic products,analyzed the effects and effects of different conditions on the biological degradation of estrogen in water,and established a feasible scheme for biodegradation.It provides theoretical basis and technical methods for the biological method of estrogen-contaminated water body repair and control of aquatic product safety.