| Solid lipid nanoparticle delivery systems are emerging delivery systems in the 21 st century that employ single or mixed solid lipids,such as lecithin,glyceryl monostearate,stearic acid and so on,as the oil phase.The oil phase is dispersed in the aqueous phase and form a uniform and stable delivery system by ultrasonic,microjet,high pressure homogenization techniques.The solid lipid nanoparticle delivery system is widely applied in sustained release system of a fatsoluble active substance based on solid lipid as a carrier of a biologically active component.In addition,the delivery system has attracted more and more attention in the food,medicine,cosmetics and other industries because of green safety,biodegradability,and biodegradability of enhanced substances advantages.Recently,triglycerides,a common carrier in solid lipid nanoparticle delivery systems,are relatively easy to accumulate in the human body resulting in diseases such as arteriosclerosis.While diglycerides,a food additive,can be instead of triglycerides to reduce the concentration of triglycerides in foods,thereby preventing the occurrence of diseases such as arteriosclerosis.Furthermore,diglycerides can also be absorbed through the lymphatic system and the portal system and reduce the burden on the gastrointestinal tract which can be an energy supply for gastrointestinal dysfunction patients.In addition,diglycerides can be quickly metabolized to avoid accumulation in the body and reduce the risk of obesity.Ferulic acid,an important natural antioxidant,can effectively scavenge free radicals and mediate immune function which has been widely applied in medicine,food,cosmetics and other fields.However,the stability of ferulic acid can be easily changed by environmental factors such as temperature and pH,which limits the its application.In this research,we fabricate the ferulic acid solid lipid nanoparticles by ultrasonic method and investigate the optimized amount of ferulic acid according to evaluating effects of ultrasonic time,ultrasonic power,oil-water ratio and pH on stability.And compared the particle size and potential,of digestion rate and biodegradability of ferulic acid,as the core material,in solid lipid nanoparticles,corn oil and flax oil emulsion delivery system.The mainly content as follows:(1)A glyceride containing glyceryl stearate diglyceride is synthesized by an enzymatic method and chemically deacidified is carried out.The composition,melting point,crystal morphology,microstructure,solid fat content and other properties were observed and determined.The results showed that the stable ? crystal form can be synthesized after 60 min reaction at65 °C by 435 enzyme catalyzing the reaction of citric acid,stearic acid and glycerol.The meltingpoint of crystal stable ? crystal product is 38.78±0.47 ? and content of glycerol the ester is41.68±0.24%,and the catkins were observed under an optical microscope.(2)The effects of ultrasonic time,ultrasonic power,oil-water ratio and pH on the stability ewof idling solid lipid nanoparticles were investigated by single factor test.The results showed that glyceryl citrate was used as raw material and SLNs were prepared by ultrasound method.The optimized condition is that ultrasonic condition was 5 min with 5 s intermittence,the ultrasonic power was 180 W,the water-oil ratio was 70,and the suitable addition amount of ferulic acid was 5%(w/w).The particle size of FA-SLNs is 145.00±2.31 nm,a zeta potential is-36.00±1.54 mV,the PDI is 0.30±0.02 and an entrapment efficiency of 91.50% ±1.27 at optimized condition.FA-SLNs is spherical under transmission electron microscopy.(3)Investigation the effects of different pH values on the particle size,zeta potential and PDI of SLNs system.The results show that when the pH value is 4 or 5,the Zeta potential of SLNs system is lower than-25 mV,and the dispersion index PDI is lower than 0.4.The whole system is in good dispersibility and stability.The size of the particles is the smallest,and the solid lipid nanoparticle suspension is a uniformly stable milky white transparent liquid.the solid lipid nanoparticle delivery system is not stable when the pH beyond 4 or 5.(4)Fabrication the ferulic acid loaded delivery system based on different oils.Finding that the bioactivity of ferulic acid solid lipid nanoparticles with stearic acid decanoate as wall up to55% while the bioavailability of ferulic acid emulsion with corn oil as wall is up to 80% after simulated digestion in vitro and the ferulic acid emulsion prepared by corn oil has the fastest digestion rate.The particle size of ferulic acid emulsion and ferulic acid solid lipid nanoparticles was increasing and Zeta potential decreasing after simulated oral cavity and stomach digestion while presenting opposite tendency after simulated the intestinal digestion. |
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