| Zinc is the second most abundant transition metal element in the human body and plays an important role in a variety of biological processes such as gene expression,neural signal transmission,DNA binding or recognition.In addition,the excessive release of zinc in different industrial processes such as mining,refining,and waste combustion will also decrease of soil microorganisim,leading to plant death.Therefore,efficient,fast,and convenient detection of Zn(Ⅱ)becomes critical.Among many detection technologies,fluorescence analysis technology is simple,economical,fast,and sensitive.It is currently the most competitive detection technology in the field of analysis and detection.At present,the fluorescent probes for Zn(Ⅱ)detection have been rapidly developed based on the fluorescence detection technology,but most of the probes have the disadvantages of low water solubility,poor selectivity,complicated preparation,and insignificant fluorescence response mode.Therefore,development of Zn(Ⅱ)fluorescent probes with simple preparation,low cost and multimode responsive has attracted widespread attentions from researchers.In this dissertation,two kinds of Zn(Ⅱ)fluorescent probes with different response modes have been developed,and the recognition behavior of Zn(Ⅱ)probes have been systematically studied,and preliminary application research has been explored.This academic thesis includes the following four chapters.The first chapter: The importance of Zn(Ⅱ)detection,detection mechanism and research progress of Zn(Ⅱ)detection based on fluorescence technology are briefly described.The second chapter: A Zn(Ⅱ)fluorescent probe is developed based on the fluorescence wavelength change responsive mode.The probe consists of 8-hydroxyquinoline and coumarin structural units.Among them,the coumarin unit is an electron donor,and the quinoline unit is an electron acceptor,forming a typical electronic "push-pull" structure.When the probe interacts with Zn(Ⅱ),the electrons are transferred from the coumarin unit to the quinoline unit under the excitation of light,resulting in a significant red shift in the ultraviolet absorption and fluorescence emission spectrum of the probe.Due to the appropriate binding space and strong binding ability with Zn(Ⅱ),the probe shows good selectivity and sensitivity to Zn(Ⅱ).In addition,the test paper prepared from the probe solution has obvious visual detection ability for Zn(Ⅱ),and has potential application value.The third chapter: A solvent-regulated Zn(Ⅱ)fluorescent probe was developed based on the response pattern of fluorescence intensity changes.The probe is a Schiff base derivative,which is obtained by a condensation reaction of tris(2-aminoethyl)amine and salicylaldehyde.By changing the polarity of the solvent,the selectivity of the fluorescent probe can be changed from poor to excellent,and it finally recognizing Zn(Ⅱ)with high selectivity.By means of nuclear magnetic resonance technology,mass spectrometry,and theoretical calculations,the binding mode of the probe with Zn(Ⅱ)ions is explored.The probe can be used not only for the detection of solutions and actual samples,but also for the detection of Zn(Ⅱ)in biological systems.In addition,it is found that the fluorescence emission of probe can be regulated by Zn(Ⅱ)and Cu(Ⅱ).The fourth chapter: Summary and prospects. |
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