Inhibition Of Acrylamide By Herba Peristrophe Japonica Extracts In Simulated System

The formation of acrylamide in high-temperature processed foods has attracted more and more attention.Because of its carcinogenic,neurotoxic and reproductive toxicity,a class ? carcinogen,and its high content in high-temperature heated foods such as French fries,fries,coffee and so on,in which starch,carbohydrates and other food materials are rich,so its content control has very important significance.Therefore,it has become a research hotspot of scientists to search for safe and efficient control measures for reducing the production of acrylamide in hot-processed food and promoting its digestion and reducing its content,reduce the exposure of acrylamide in people's food.In this paper,the inhibition and dose-effect relationship of the extracts of Herba Peristrophe japonica on acrylamide in asparagine/glucose simulated system under low-wet roasting mode were studied.The formation/elimination kinetics model of acrylamide in the system was established and analyzed,and the formation mechanism of acrylamide was preliminarily expounded.The main research contents and results were as follows:?1?The inhibitory regular pattern and dose-effect relationship of the extracts on acrylamide in asparagine/glucose simulation system were studied,which including the 95%ethanol extract of Herba Peristrophe japonica,and petroleum ether extract,ethyl acetate extract,n-butanol extract and water extract.Add 100?L of each extract to the simulated system?asparagine:glucose=1.4 mmol:1.4 mmol?and bake at 180?for 15 min to measure the acrylamide content.The acrylamide contents of each group were detected.The results showed that 95%ethanol extract and different solvent extracts had inhibitory effects on the acrylamide content,and their concentration had a non-linear relationship with acrylamide content.The inhibitory effects of the extracts were in the order of large to small as follows:the n-butanol extract,ethyl acetate extract,water extract,petroleum ether extract,and 95%ethanol extract.Their optimum inhibitory concentrations and inhibitory rates were 3 mg/mL and 56.78%,5mg/mL and 47.96%,1×10^-55 mg/mL and 47.26%,4 mg/mL and 46.49%,1×10^-9mg/mL and 41.22%respectively.?2?It was further studied that the inhibitory regular pattern and dose-effect relationship of different ethanol eluents on acrylamide in the simulation system,obtained by separated from the n-butanol extract with the best inhibitory effect of acrylamide.The results showed that the 95%and 60%ethanol eluents had better inhibitory effects on acrylamide.The inhibitory effects in turn from large to small were 95%,60%,30%ethanol eluent.Their optimum inhibitory concentrations and inhibitory rates were 5 mg/mL and 64.20%,1×10^-22 mg/mL and 61.99%,1.0×10^-9mg/mL and 26.50%respectively.It was still non-linear relationship between the concentration of three ethanol eluents and the acrylamide content.The inhibition rate of 95%ethanol eluent at high concentration was higher than that at low concentration,its optimal inhibition concentration was 5 mg/mL.Inhibitors with better inhibitory effect of acrylamide can be separated and extracted from this part.?3?Under the action of 5 mg/mL 95%ethanol eluent of in simulation system,the formation/elimination kinetic of acrylamide was studied,which having the highest inhibition rate of acrylamide.The results showed that the formation rate of acrylamide was the fastest from the 5th min to 7th min in the 95%ethanol eluent group,the acrylamide production reached the maximum at the 8th minute,and the maximum was at the 7th minute in the control group,a little earlier than the former.After reaching the maximum,it showed a significant downward trend of acrylamide content in these two groups.There was a significant difference between the test group and the blank control group?p<0.05?.The fitting of the kinetic curve of acrylamide content was applied with Origin 9.1.According to the fitting curve of GaussMod model,the R² value of blank group and test group were 0.9597 and 0.9439 respectively,which indicated that the GaussMod model could predict acrylamide kinetics on the condition of 5 mg/mL 95%ethanol eluent in asparagine/glucose simulation system under low-humidity baking mode,which was of great significance for guiding the prediction of acrylamide content under the condition of adding 95%ethanol eluent in simulation system.?4?By studying the changes of the content of asparagine,glucose,fructose,acrylamide and melanoids in the baking time of 0-20 min which were the precursors,intermediate and their final product formed throughout the reaction process,the kinetic curves of them were drawn respectivelyto explore the mechanism of inhibition of acrylamide by ethanol extract of n-butanol extract.The results showed that the isomerization of glucose to fructose was the main reaction from the zeroth min to the5th minute,and a small amount of melanoids was formed in Maillard reaction in the asparagine/glucose simulation system under the low wet roasting mode.There was no correlation between melanoids to acrylamide in this stage.After the 5th minute,a large amount of acrylamide through acrolein pathway produced by the reaction of glucose and fructose with asparagine,and then a large amount of melanoids were produced.At this stage,the formation of acrylamide was related to the formation of melanoids.At the same time,95%ethanol eluent could inhibit acrylamide production and generation time significantly.After the 8th minute,the acrylamide elimination became greater than the formation,and the formation of melanoids was still rising rapidly.The 95%ethanol eluent had no obvious effect on the elimination of acrylamide.The removal rate of acrylamide was much higher than the formation rate at the 15th min to the 20th min,and the content of melanoids maintained stable at the maximum.There was no correlation between them at this stage.The formation mechanism of acrylamide is closely related to the isomerization of glucose,the acrolein pathway and Maillard pathway of the reaction of glucose and fructose with asparagine.The 95%ethanol eluent can affect the reaction in simulated system,thus affecting the amount and time of acrylamide formation.