The Preparation Of Mesoporous Carbon And Its Composite Applications As Carriers For Drug Delivery

Mesoporous carbon material is one of important members of functional nano materials.Due to its advantages such as porous nanostru cture,large surface area,excellent permeability,morphological diversity and functional shell,it is widely applied to various fields.Therefore,mesoporous carbon composites,combining high drug loading capacity,large surface area,functional shells an d inner voids and pretty good biocompatibility,had become the preferred drug carrier materials for researchers.The mesoporous Fe₃O₄ nanoclusters were synthesized via solvothermal method.The raw materials were ferric chloride hexahydrate?FeCl₃·6H₂O?and anhydrous sodium acetate?CH₃COONa?,ethylene glycol?EG?and diethylene glycol?DEG?working as dispersing agents and reducing agents,and polyvinylpyrrolidone?PVP K-30?serving as surfactant.The mesoporous Fe₃O₄ was prepared and characterized by scanning electron microscopy?SEM?,transmission electron microscopy?TEM?,Fourier infrared spectrometer?FTIR?Brunauer-Emmett-Teller?BET?.Meanwhile,we also studied some key factors,such as reaction time,reaction temperature and the mass of CH ₃COONa.The study found that the 100 nm uniform mesoporous Fe₃O₄ was obtained with the reaction conditions of 0.54 g FeCl₃·6H₂O,1.5 g CH₃COONa,at 200?for 12 h.What's more,its specific surface area was 79.47 m²?g^-1 and the average pore size was12.35 nm.Therefore,the magnetic microsphere can be applied into drug loading and releasing.The obtained Fe₂O₃ microspheres were used as templates and coated with dopamine to form the dopamine@Fe₃O₄ microspheres,followed by annealing to yield C@Fe₃O₄ and then acid etching to obtain mesoporous carbon.The characterization of the mesoporous carbon showed that the specific surface area was 578.91 m²?g^-?16?,and the average pore size was 6.9 nm.Besides,the saturation magnetization strength changed from 60.19 emu?g^-?16?of C@Fe₃O₄ to 1.12 emu?g^-?16?of mesoporous carbon,implying the mesoporous carbon retained magnetic property and could work as targeted drug carriers.When the drug concentration was 0.5mg?cm^-3 of 5-Fluorouracil?5-Fu?,the loading capacity of magnetic mesoporous carbon was reached to 44%.And the drug cumulative releasing amount was 38.5%under the slightly acid condition.In addition,the magnetic mesoporous carbon had high performance of drug capacity and drug releasing.Therefore,magnetic mesoporous carbon can be used as a target carrier.Another mesoporous carbon was prepared by soft template.The Z IF?like zeolite struture?was prepared by hydrothermal method.Then,the SiO₂ was coated on the ZIF surface.And the mesoporous carbon was obtained by high temperature calcination.Finally,the poly?acrylic acid??PAA?was grafted on mesoporous carbon surface.The results showed that the specific surface area of mCNC@mSiO₂was 545.9 m²?g^-1,and the average pore size was 7.9 nm.The Hela cells viability was above 95%in different concentrations of mCNC@mSiO₂@PAA.Hence,the mCNC@mSiO₂@PAA was low toxicity,and had quite bio-safety.And the drug loading capacity of 5mg mCNC@mSiO₂@PAA and 5 mg mCNC@mSiO₂ was1.009 mg and 0.723 mg,respectively.The releasing experiments showed that mCNC@mSiO₂ exhibited a low loading capacity and a fast releasing speed relatively during same time.While the releasing of mCNC@mSiO₂@PAA was more durable and the high loading capacity was relatively high.T herefore,MOF-derived has a better prospect of biological application.