The Synthesis Of Fluorescent Nanomaterials And Its Application In Biosensors

Persistent organic pollutants(POPs)are persist in the nature environment,can be cause potential hazards to human health and environment.With the rapid development of modern science and technology,more and more POPs are released into the environment,increasingly making it a global environmental problem.Tris(2,3-dibromopropyl)isocyanurate(TBC)is a novel POPs and has high thermal stability,durability,and resistance to photo degradation.At present,the detection of TBC depends on instrument methods like HPLC,MS and HPLC-MS.But these analysis methods are generally expensive,time-consuming,and labor-intensive and also need expensive large instruments,require complex sample pretreatments,that will restrict their widespread application for rapid and real-time analysis in environmental studies.Therefore,it is of great significance to develop a highly sensitive,selective and simpler analytical method for quick analysis of TBC.Based on excellent fluorescent properties of fluorescent nanomaterials,we developed a rapid analysis of TBC,which may bring new ideas for the rapid analysis of POPs.Two works were presented as below in this article:(1)Two TBC haptens OVA-TBC and BSA-TBC were prepared.Utilizing OVATBC as immunogens in BALB/c mice to produce TBC monoclonal antibody(TBC mAb)by induction method.The TBC mAb was proved to be sensitive for TBC and could be further used in immunoassay.(2)Based on competitive immunoassay,we now construct a colorimetric and fluorescent dual-mode lateral flow immunoassay(LFIA)for rapid detection of TBC: Gold conjugate was dispensed onto the conjugate pad,while the FITC-BSA-TBC conjugates and the goat anti-mouse IgG antibody were separately dispensed onto the nitrocellulose membrane as test and control line.If TBC are present in the sample,goldTBC mAb conjugates will bind them and the FITC-BSA-TBC conjugate immobilized on test line fail to bind to TBC antibody.The goat anti-mouse antibody on the control line captures any excess gold particlethus producing a visible line of deposited gold in control line.When there is no TBC in sample,gold TBC antibody conjugates will be captured by the test line as they traverse the membrane,lead to quench the fluorescence of FITC.The decrease of the test line is directly correlated to the increase of the amount of TBC in the sample.This assay allowed the linear detection of TBC by color ranging from 5 to 60 ?g/L,with a LOD value of 5 ?g/L.while the detection of TBC by color ranging from 1 to 50 ?g/L,with a LOD value of 1 ?g/L.The results can be judged within 15 min.The strip test had a lower LOD by using the FITC against visual detection.Having advantages of high sensity and accuracy,this method is expected to be used in rapid analysis of TBC in actual samples.(3)we developed a facile strategy for one-step hydrothermal synthesis of CDs with surface passivation by using EDTA and 3-APBA.Based on Photo-induced Electron Transfer(PET)between dopamine and CDs,which the electron transform from CDs to dopamine,the fluorescence of CDs was quenched.This method has a linear range of 0.05 to 10 ?M.The detection limit was found to be as low as 0.015 ?M.Considering the advantages of good selectivity,high sensitivity,eco-friendly,easily operation and rapidly detection,we expect this method to play an important role in biological and environmental applications.