Determination Of Organic Acids And Thiols Content In Seminal Plasma Of Patients With Hyperuricemia And Its Clinical Value

In recent years,with the changes in people's material living standard and diet habits,the incidence of hyperuricemia(HUA)has shown a significantly increased trend in the world.A large number of studies have shown that the prevalence of hyperuricemial in men are significantly higher than before,and the male patients with hyperuricemia tend to be younger.Elevated uric acid levels can cause metabolic disorders and various syndromes.Therefore,hyperuricemia will affect male reproductive health to a certain extent.In the same period,the quality of men's semen has been declining globally.As a special and unique part of the male body,seminal plasma has a significant impact on male reproductive health.The important components of seminal plasma such as organic acids and thiols reflect their functional health from the perspective of redox.Most of the studies on organic acids and thiols are done on human blood,but there are few studies on the determination of organic acids and thiols in seminal plasma and its clinical significance.This article uses ultra high performance liquid chromatography(UPLC)to determine the content of organic acids and thiols in the seminal plasma of normal people and patients with hyperuricemia and discuss their clinical significance.The thesis includes the following two parts:1.To investigate the changes and clinical significance of the contents of seminal plasma organic acids in men between the two groups of normal population and hyperuricemia patients.Methods RP-HPLC was used to determine citric acid,ascorbic acid,succinic acid,tartaric acid,lactic acid and uric acid level,analysis level changes between the two groups.At the same time,the degradation rate of six organic acids at different temperatures,the relationship between six organic acids in seminal plasma and semen parameters in normal people,and the correlation between seminal plasma uric acid and blood uric acid in patients with hyperuricemia were investigated.Results ?The ascorbic acid,tartaric acid,lactic acid and uric acid content in the HUA group were significantly lower than that in the normal group(p <0.05),and the citric acid and succinic acid content showed a decreasing trend compared with the normal group;?When placed at 30?,the degradation rate of the seminal plasma samples is the fastest,the degradation rate is moderate at 4?,and it is relatively stable when stored at-20?;?In normal population,there is no correlation between the content of 6 organic acids and their corresponding semen volume,sperm concentration and sperm motility(P> 0.05);?There was no correlation between seminal plasma uric acid and blood uric acid in patients with hyperuricemia,the correlation coefficient r = 0.129,P = 0.243.Conclusion The content of organic acids in seminal plasma of patients with hyperuricemia has significant changes compared with the normal group,suggesting that the redox function of patients with hyperuricemia is impaired.2.To investigate the changes and clinical significance of seminal plasma content in men between the two groups of normal population and patients with hyperuricemia.Methods The levels of homocysteine(Hcy),cysteine(Cys),cysteinylglycine(CysGly)and glutathione(GSH)in seminal plasma were determined by RP-HPLC with fluorescence detection.Results There were significant differences in the content of four thiols substances in the HUA group compared with the normal group(P < 0.05),in which the levels of Cys,Hcy and GSH increased,while the levels of CysGly decreased.Conclusion The content of thiols in seminal plasma of patients with hyperuricemia is significantly changed compared with the normal group,suggesting that the redox function of patients with hyperuricemia is impaired.