A Study On Constructing Nanofiber Composite Hernia Patch Based On P(CL/LLA)/Porcine Fibrin

Hernia is a general surgical disease that has a high incidence and can only be treated by surgery.At present,tension-free hernia repair is commonly used,which uses patch materials to repair the hernia ring.The popularization of hernia repair patches in the clinic has greatly reduced the pain and recurrence of hernias,but some of the patch materials cannot be degraded and absorbed,which will cause foreign bodies and uncomfortable reactions.However,the absorbable material is easy to cause defects due to the lack of the ability to promote the regeneration of the abdominal wall.These conditions will cause some new complications.In order to construct a degradable and absorbable hernia repair patch with suitable mechanical properties and capable of inducing tissue regeneration,this article first investigated the electrospinning parameters by adjusting the spinning solution concentration,spinning voltage,electrospinning distance and perfusion speed Influence on the surface morphology of[poly(?-caprolactone-co-L-lactide),P(CL/LLA)] membrane.Then,the P(CL/LLA)solution with a concentration of 9 % and the pig-derived fibrin solution were blended and spun according to a volume ratio of 1: 0,2: 1,1: 1,1: 2,0: 1 to prepare a A series of P(CL/LLA)/Porcine fibrin composite electrospun membranes were used to evaluate the apparent morphology of the samples using SEM photographs of electrospun membranes combined with porosity measurements;they were characterized by FITR and XPS The groups and elements on the surface of the composite electrospun membrane;the hydrophilicity of the material was evaluated by the static water contact angle experiment;the mechanical properties and biocompatibility of the P(CL/LLA)/Porcine fibrin composite electrospun membrane The performance was tested and evaluated;finally,in theaspect of product quality control,subsequent dimensional processing and solvent volatility testing were also carried out according to relevant standards.The research results show that the P(CL/LLA)nanofiber material prepared by electrostatic spinning technology has a microstructure similar to that of natural ECM,and its morphological structure changes regularly with the setting of different parameters: the diameter of the electrospun fiber will follow the spinning The concentration of the liquid becomes thicker and the optimal spinning concentration of P(CL/LLA)is 6 %~9 %;the fiber diameter will become smaller as the voltage increases;the receiving distance is too short or the perfusion is too fast As a result,the solvent is difficult to volatilize sufficiently,causing adhesion between the fibers.P(CL/LLA)and pig-derived fibrin can be combined into a material with high porosity and good pore connectivity by electrospinning technology,and no chemical reaction occurs between the two substances.P(CL/LLA)/Porcine fibrin composite electrospun membrane is not toxic to cells,and as the proportion of porcine fibrin gradually increases,the fiber diameter of the electrospun membrane gradually decreases,porosity increases,and hydrophilic properties And the performance of promoting cell adhesion and proliferation has been improved,but its mechanical properties such as tensile resistance,bursting,tearing and suture pulling have declined to varying degrees.By comparing the performance results of various groups of electrospun membranes,it is found that when the volume of P(CL/LLA)/ Porcine fibrin is 2: 1,the cell compatibility and the ability to induce cell growth and proliferation are the best,and the mechanics The performance can also meet the standards of clinical use,and has the potential to induce tissue regeneration.In addition,P(CL/LLA)/Porcine fibrin composite hernia repair sheet can be perforated to obtain a tight and durable fixation after implantation.The size of the mesh will be reduced to a certain extent within6 to 10 days after perforation.Therefore,the size should be adjusted to make it fully stable before delivery.Finally,the HFIP residue of 35 angel mesh should be less than 0.55 mg /g of the minimum standard,and it should be irradiated and sterilized at the same time and stored in a dark vacuum at 2~8 ?.