Preparation Of Polyacrylonitrile Nanofibers Loaded With Fe₂O₃ Nanoparticles And Construction Of A Glucose Biosensor

In order to achieve direct electron transfer between the enzyme and the electrode,a glucose biosensor having high sensitivity,high selectivity,and strong anti-interference performance is obtained.In this paper,polyacrylonitrile?PAN?nanomaterials were prepared using electrospinning technology that is easy to operate and can efficiently prepare nanomaterials.Preparation of Ferric Oxide?Fe₂O₃?nanoparticles with high purity and uniform size by hydrothermal synthesis,by controlling the pH of the hydrothermal reaction to adjust the cohydrolysis strength of the reactants,Fe₂O₃ nanoparticles were controlled to adhere on the surface of PAN nanofibers,and PAN/Fe₂O₃ composite nanofibers with uniform dispersion of Fe₂O₃nanoparticles on the surface of PAN nanofibers were obtained.The chitosan/silica?CS/SiO₂?gel prepared by the sol-gel method is often used for the immobilization of enzymes because of its good biocompatibility and ability to keep the activity of the enzyme well.However,the gel prepared by this method has the disadvantages of film cracking and poor continuity.In this paper,PAN/Fe₂O₃ composite nanofibers were introduced on the basis of the sol-gel method to obtain a good and continuous composite gel.It is used to immobilize glucose oxidase and prepare nano-glucose biosensor based on nanomaterials.The morphologies of PAN nanofibers were characterized by scanning electron microscopy?SEM?,the effects of electrospinning process parameters on the preparation of PAN nanofibers were studied;Scanning electron microscopy?SEM?,infrared spectroscopy?FT-IR?,thermogravimetric analysis?TG?and X-ray diffraction?XRD?were used to characterize the PAN/Fe₂O₃ composite nanofibers,the dispersion of nanoparticles was studied to verify that the coating mechanism of Fe₂O₃nanoparticles dispersed and bonded on the surface of PAN nanofibers was cohydrolysis;Scanning electron microscopy?SEM?was used to observe the surface morphology of gels with composite fibers prepared by sol-gel method,the effect of PAN nanofibers on gelation properties and film continuity was studied;The electrochemical performance of the working electrode was tested by cyclic voltammetry and chronopotentiometry,the catalytic oxidation performance and sensitivity of the prepared glucose biosensor were studied.The results of this study indicate that the preparation of PAN nanofibers is influenced by multiple factors such as spinning parameters,material properties,and external environment;The intermediates produced during the hydrothermal synthesis of Fe₂O₃ nanoparticles can activate the hydrolysis of PAN,which generates Fe₂O₃nanoparticle binding sites on the surface of the fibers by hydrolysis.The strength of the hydrolysis is regulated by the hydrothermal pH,nanoparticles and nanofibers are effectively covalently bonded to overcome the problem of easy reunion of nanoparticles;The PAN/Fe₂O₃ composite nanofibers effectively improved the morphology of the gel prepared by the sol-gel method,so that the gel became continuous and conducive to the signal transmission between the enzyme and the electrode surface;Fe₂O₃ nanoparticles dispersed in PAN nanofibers accelerate electron transfer between enzymes and electrodes;Nano-glucose biosensors based on Fe₂O₃ nanoparticles,PAN nanofibers,and sol-gel methods have better catalytic oxidation performance and sensitivity.