Explore On The Extraction Technology Of Pear Cactus And Fenugreek And Its Mechanism Of Preventing Alcoholic Brain Injury

Objective: Chronic alcoholism is one of the global public health problems,long-term excessive drinking is one of the causes of many chronic diseases.The mechanism of alcohol-induced liver injury and its drug research have been very mature,but there are few reports on the mechanism of alcohol-induced brain injury.The damage of alcohol to the body cells is mainly achieved by inducing oxidative stress of cells and regulating the activity of various enzymes in mice.Excessive drinking for a long time will lead to the changes of SOD,GSH and MDA enzyme activities in cells of the body,and further lead to oxidative stress of cells and produce neurotoxin to damage the health of central nervous system.Pear cactus and fenugreek have a long history of medicinal use.They have good antioxidant activity,increase ADH activity,and regulate the metabolism of neurotransmitters in the brain.This study is devoted to explore the preventive effect of pear cactus and fenugreek extract on alcoholic brain injury.Method: 1.Research on extraction process: The effects of ethanol extraction concentration,material-liquid ratio,and microwave extraction power on the extraction rate of polysaccharides of pear and cactus polysaccharide and the ratio of material-liquid ratio,number of extractions,and extraction time were studied using response surface optimization Effect on the extraction rate of fenugreek flavonoids 2.Establish animal experimental model of alcoholic brain injury: use a kit to measure the activity of various enzymes involved in alcohol metabolism in rats,including AST(aspartate aminotransferase)in rat blood,Activity of SOD(superoxide dismutase),GSH(reducing glutathione enzyme),MDA(malondialdehyde),AchE(acetylcholinesterase)in mouse ALT and glutamine aminotransferase.Further determine the activity of neurotransmitters in the brain of mice such as 5-HT(serotonin),NE(norepinephrine),DA(dopamine)and so on.And through the software analysis of the measured data to determine the long-term excessive alcohol damage to the brain nerves in mice.Alcohol blood drug concentration time curve is used to visualize the alcohol metabolism of rats in different groups.3.Based on the experimental model of drunkenness,compare the experimental results of different administration groups to analyze the preventive effect of fenugreek and pear cactus on alcoholic brain injury.Results: 1.Response surface optimization to obtain the best extraction process: The optimal extraction process of pear and cactus polysaccharides was ethanol concentration70%,material-liquid ratio 1:10,microwave power 450 w,and the extraction rate of cactus polysaccharides was 8.45%.The best extraction process of fenugreek flavonoids is the material-liquid ratio of 1:4,the extraction time is 1.5h,the extraction frequency is 3 times,andthe extraction rate is 2.01% after repeating the experiment 3 times.3.Mouse alcoholic brain injury model experiment: compared with the blank group,the model group had higher AST /ALT,liver coefficient,brain coefficient,brain SOD,GSH activity was inhibited,MDA content increased,brain DA,5-The content of HT and NE decreased,and the activity of AchE enzyme decreased and all had significant differences(p <0.05).4.Fenugreek flavonoids and pear fruit cactus prevent alcoholic brain injury experiment: compared with the model group,fenugreek flavonoids,pear fruit cactus polysaccharide high-dose group and low-dose group AST / ALT decreased,organ coefficient decreased,brain The activity of SOD and GSH increased in MDA,while the content of MDA decreased,while DA,5-HT,NE in brain increased,AchE decreased,and the ratio of high-dose group to model group was significantly different(p<0.05).