Design,Synthesis And Properties Study Of Reaction-based Thiols And Hydrogen Peroxide Flourescent Probes

The fluorescent probe detection method is widely used in the field of analysis and detection.This is due to its high selectivity,high sensitivity,fast and convenient,and less harm to the sample.The detection occupies an important position,so the synthesis of fluorescent probes that can detect them efficiently,quickly,and with good selectivity has become the focus of fluorescent probe research.Biothiols mainly include glutathione?GSH?,cysteine?Cys?and homocysteine?Hcy?,which play an important role in human physiological activities.The reported biological thiol fluorescent probes still have some shortcomings.For example,the response time to thiol is longer,and the reaction time is generally more than 30 min;or the probe is greatly affected by pH and is not suitable for detection in a physiological environment..In the second chapter of this article,the fluorescent probe N-n-butyl-6-acrylate-anthracene imide?NA-1?And fluorescent probe?NA-2?with lysosomal localization effect.The detection results of biomercaptan in DMSO-PBS system showed that only 5.0 min reaction time was needed to reach the maximum fluorescence response value,the fluorescence intensity at 601 nm was significantly increased by 121 times,and the color of the solution could be observed with naked eyes The change shows that the probe has a dual-channel detection function.The probe?NA-1?has a high selectivity for biomercaptans,with a minimum detection limit of 0.87?M.It can be used for linear testing of biomercaptans in the range of 10.0-1000?M and has been successfully applied to cell testing.As an important reactive oxygen species?ROS?,hydrogen peroxide plays a vital role in many physiological and pathological processes.In the literature reported so far,most of the hydrogen peroxide fluorescent probes use fluorescein,naphthalimide,and coumarin as fluorophores,which mainly have the defect of long reaction time,which generally takes more than 60.0 min.In the third chapter of this paper,the fluorescent probe N-n-butyl-6-boronate-anthracene imide?NABO?was designed and synthesized by triggering the reaction mechanism between borate and H₂O₂ using anthracene as the matrix),Excellent detection performance for hydrogen peroxide in DMSO-PBS system.The probe NABO can reach the maximum fluorescence in 30.0 min in the presence of hydrogen peroxide?H₂O₂?,and the fluorescence increases by 18 times,which is basically not affected by pH.The minimum detection limit is 47.0?M,in the physiological range of 0.1-10.0 mM Linear detection of hydrogen peroxide.In summary,this paper developed a new class of fluorescent probes for detecting biomercaptan and hydrogen peroxide.The detection time is greatly shortened and less affected by the detection environment.It provides a new method for the detection of biomercaptan and hydrogen peroxide in humans way.