Gold-doped carbon dots(CDAu)were simply and quickly prepared by microwave method using fullerene as a precursor,and were characterized in detail.It was found that CDAu has a strong catalytic effect on the nano-reaction of reducing chloroauric acid to nano-gold?AuNP?by glucose.The nano-reaction was studied by SERS,RRS,and fluorescence.After adding As???nucleic acid aptamer(AptAs)to the nano-reaction system,the carbon dots are wrapped by the aptamer,and the carbon dot catalysis is weakened,resulting in a decrease in AuNP formation and a decrease in the SERS intensity.When the target molecule As???is added,it specifically binds to AptAs to release CDAu and restore its catalytic activity.The SERS strength of the system is enhanced.Within a certain range,the degree of enhancement has a linear relationship with the concentration of As???.Based on this,a new SERS method for quantitative determination of 0.04-0.23?g/L As???has been established with a detection limit of 0.02?g/L.AptAs-As???-CDAu-glucose-HAuCl4 system RRS and photometric determination of As???have linear ranges of 0.06-0.38?g/L and0.03-0.2?g/L,and the detection limits are 0.03?g/L and 0.01?g/L.The linear range and detection limit of As???by AptAs-As???-CDAu-Dopamine-HAuCl4 system were0.1-0.6?g/L and 0.04?g/L.Based on the AptHg-CDAu-Hg?II?-glucose-HAuCl4system,a new SERS analysis method was developed for the simple and rapid determination of 2-12?mol/L Hg?II?,with a detection limit of 0.9?mol/L.Based on the AptPb-CDAu-Pb?II?-glucose-HAuCl4 system,a new SERS analysis method was developed for the simple and rapid determination of 1.7-13.3 nmol/L Pb?II?with a detection limit of 0.8 nmol/L. |