Electrochemical Sensing Of Nucleic Acid Aptamers Based On ZIF-8 Derived Porous Carbon Materials

Porous carbon materials derived from MOFs have attracted much attention due to their excellent properties.They not only inherit the advantages of MOFs,such as high surface areas,adjustable pore structure,and large pore capacity,but also derive excellent electrochemical performance,high biocompatibility,and good thermal stability.These outstanding properties make it widely used in supercapacitors,catalysts,and electrochemical sensing.Among them,ZIF-8 has the advantages of high carbon content,excellent stability,and ordered pore structure.It can be directly carbonized into nanoporous carbon materials without adding other carbon sources.The preparation steps are simple and efficient.Therefore,we used ZIF-8 as a precursor to study the performance of its derived porous carbon at different carbonization temperatures and its application in the electrochemical biosensing of nucleic acid aptamers.We designed a homogeneous electrochemical aptamer sensor for the thrombin detection based on the surface structure and excellent electrical conductivity of ZIF-8 derived porous carbon.ZIF-8 was carbonized at 1000 °C to obtain its derived porous carbon Z-1000.Z-1000 shows a large surface area and excellent electrochemical performance.The addition of Z-1000 not only improves the detection sensitivity and electron transfer efficiency of the sensor,but the existence of its porous carbon nanostructures also effectively improves the adsorption of nucleic acid aptamer chains and the selectivity of the sensor.The results showed that the Z-1000-based biosensor has high sensitivity,good selection,and fast response to target detection.And the sensor could be successfully applied for the actual samples detecting.We designed an electrochemical biosensor based on the pore structure of ZIF-8 derived porous carbon for stimulus-responsive nanocontainer thrombin detection.At 700 °C,Z-700 was used as a porous nanocontainer loaded with guest molecules.The uniform pore structure and large pore capacity of Z-700 make the prepared nanocontainer have higher loading efficiency for guest molecules.At the same time,the strategy that carbon nanostructures can be combined with oligonucleotides significantly improves the formation efficiency of DNA gating and simplifies the encapsulation process of guest molecules.And the interaction of biomolecules enables controlled release of guest molecules.This solution developed the application of MOFs-derived porous carbon in stimulus-responsive nanocontainers biosensing,and provided new ideas and methods for the detection of other analytes.